RNAi knockdown of transcription factor Pu.1 in the differentiation of mouse embryonic stem cells.

Gang Ming Zou, Meredith A. Thompson, Mervin Yoder

Research output: Contribution to journalArticle

Abstract

Murine embryonic stem (mES) cells are pluripotent cells derived from the inner cell mass of the preimplantation blastocyst. These cells are primitive and undifferentiated and have the potential to become a wide variety of specialized cell types. Mouse ES cells can be regarded as a versatile biological tool that has led to major advances in our understanding of cell and developmental biology. To study specific gene function in early developmental events, gene knockout approaches have been traditionally used, however, this is a time-consuming and expensive approach. Recently, we have shown that small interfering RNA is an effective strategy to knockdown target gene expression, during ES cell differentiation, and consequently, one can alter cell fates in ES-derived differentiated cells. This method will be useful to test the function of a wide variety of gene products using the ES cell differentiation system.

Original languageEnglish
Pages (from-to)127-136
Number of pages10
JournalMethods in molecular biology (Clifton, N.J.)
Volume407
StatePublished - 2007

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RNA Interference
Cell Differentiation
Blastocyst Inner Cell Mass
Developmental Genes
Developmental Biology
Gene Knockout Techniques
Embryonic Stem Cells
Small Interfering RNA
Genes
Cell Biology
Mouse Embryonic Stem Cells
proto-oncogene protein Spi-1
Gene Expression

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

RNAi knockdown of transcription factor Pu.1 in the differentiation of mouse embryonic stem cells. / Zou, Gang Ming; Thompson, Meredith A.; Yoder, Mervin.

In: Methods in molecular biology (Clifton, N.J.), Vol. 407, 2007, p. 127-136.

Research output: Contribution to journalArticle

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