Robust effect of metabolic syndrome on major metabolic pathways in the myocardium

Maryam Karimi, Vasile I. Pavlov, Olivia Ziegler, Nivedita Sriram, Se Young Yoon, Vahid Agbortoko, Stoiana Alexandrova, John Asara, Frank W. Sellke, Michael Sturek, Jun Feng, Boian S. Alexandrov, Anny Usheva

Research output: Contribution to journalArticle

Abstract

Although the high-fat-diet-induced metabolic syndrome (MetS) is a precursor of human cardiac pathology, the myocardial metabolic state in MetS is far from clear. The discrepancies in metabolite handling between human and small animal models and the difficulties inherent in obtaining human tissue complicate the identification of the myocardium-specific metabolic response in patients. Here we use the large animal model of swine that develops the hallmark criteria of human MetS. Our comparative metabolomics together with transcriptomics and computational nonnegative matrix factorization (NMF) interpretation of the data exposes significant decline in metabolites related to the fatty acid oxidation, glycolysis, and pentose phosphate pathway. Behind the reversal lies decreased expression of enzymes that operate in the pathways. We showed that diminished glycogen deposition is a metabolic signature of MetS in the pig myocardium. The depletion of glycogen arises from disbalance in expression of genes that break down and synthesize glycogen. We show robust acetoacetate accumulation and activated expression of key enzymes in ketone body formation, catabolism and transporters, suggesting a shift in fuel utilization in MetS. A contrasting enrichment in O-GlcNAcylated proteins uncovers hexosamine pathway and O-GlcNAcase (OGA) expression involvement in the myocardial response to MetS. Although the hexosamine biosynthetic pathway (HBP) activity and the availability of the UDP-GlcNAc substrate in the MetS myocardium is low, the level of O-GlcNacylated proteins is high as the O-GlcNacase is significantly diminished. Our data support the perception of transcriptionally driven myocardial alterations in expression of standard fatty acids, glucose metabolism, glycogen, and ketone body related enzymes and subsequent paucity of their metabolite products in MetS. This aberrant energy metabolism in the MetS myocardium provide insight into the pathogenesis of CVD in MetS.

Original languageEnglish (US)
Article numbere0225857
JournalPLoS ONE
Volume14
Issue number12
DOIs
StatePublished - Jan 1 2019
Externally publishedYes

Fingerprint

metabolic syndrome
myocardium
Metabolic Networks and Pathways
Glycogen
biochemical pathways
Myocardium
Metabolites
Hexosamines
Ketone Bodies
Animals
Enzymes
Fatty Acids
glycogen
Pentoses
Uridine Diphosphate
hexosamines
Pathology
Nutrition
ketone bodies
Factorization

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Karimi, M., Pavlov, V. I., Ziegler, O., Sriram, N., Yoon, S. Y., Agbortoko, V., ... Usheva, A. (2019). Robust effect of metabolic syndrome on major metabolic pathways in the myocardium. PLoS ONE, 14(12), [e0225857]. https://doi.org/10.1371/journal.pone.0225857

Robust effect of metabolic syndrome on major metabolic pathways in the myocardium. / Karimi, Maryam; Pavlov, Vasile I.; Ziegler, Olivia; Sriram, Nivedita; Yoon, Se Young; Agbortoko, Vahid; Alexandrova, Stoiana; Asara, John; Sellke, Frank W.; Sturek, Michael; Feng, Jun; Alexandrov, Boian S.; Usheva, Anny.

In: PLoS ONE, Vol. 14, No. 12, e0225857, 01.01.2019.

Research output: Contribution to journalArticle

Karimi, M, Pavlov, VI, Ziegler, O, Sriram, N, Yoon, SY, Agbortoko, V, Alexandrova, S, Asara, J, Sellke, FW, Sturek, M, Feng, J, Alexandrov, BS & Usheva, A 2019, 'Robust effect of metabolic syndrome on major metabolic pathways in the myocardium', PLoS ONE, vol. 14, no. 12, e0225857. https://doi.org/10.1371/journal.pone.0225857
Karimi M, Pavlov VI, Ziegler O, Sriram N, Yoon SY, Agbortoko V et al. Robust effect of metabolic syndrome on major metabolic pathways in the myocardium. PLoS ONE. 2019 Jan 1;14(12). e0225857. https://doi.org/10.1371/journal.pone.0225857
Karimi, Maryam ; Pavlov, Vasile I. ; Ziegler, Olivia ; Sriram, Nivedita ; Yoon, Se Young ; Agbortoko, Vahid ; Alexandrova, Stoiana ; Asara, John ; Sellke, Frank W. ; Sturek, Michael ; Feng, Jun ; Alexandrov, Boian S. ; Usheva, Anny. / Robust effect of metabolic syndrome on major metabolic pathways in the myocardium. In: PLoS ONE. 2019 ; Vol. 14, No. 12.
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