Rodent myoblast interactions with laminin require cell surface glycoconjugates but not laminin glycosyl groups

Tatiana Kostrominova, M. L. Tanzer

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Laminin glycosyl groups are necessary for the spreading of murine melanoma cells which become attached to this glycoprotein. Laminin has been implicated in myogenesis but the potential role of its glycosyl groups in this process has not been examined. In this study we report the effects of the carbohydrate moieties of laminin on myoblast adhesion, spreading, and differentiation. Unglycosylated laminin from tunicamycin-treated cultures of a mouse cell line, M1536 B3, was used in the experiments. Glycosylated laminin from a murine tumor and from cultures of M1563 B3 cells served as controls. Cell binding experiments with C2C12 mouse myoblasts showed that the cells preferred a laminin-coated surface, compared to the uncoated plastic surface (nontissue culture wells). Myoblasts did not distinguish between glycosylated and unglycosylated laminin substrates. Both glycosylated and unglycosylated forms of laminin promoted myoblast growth and differentiation. In contrast, cells on uncoated plastic surfaces grew very slowly and did not further differentiate. The L6 rat myoblast response to glycosylated and unglycosylated laminin was the same. These results indicate that although rodent myoblasts in culture require a laminin substratum for spreading, growth, and differentiation on a proprietary plastic surfacer laminin carbohydrates are not implicated in those cellular responses. In contrast, parallel studies using the lectin, Con A, indicate that cell surface glycoconjugates of myoblasts are implicated in the response of these cells to a laminin substratum.

Original languageEnglish (US)
Pages (from-to)163-172
Number of pages10
JournalJournal of Cellular Biochemistry
Volume57
Issue number1
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

Glycoconjugates
Myoblasts
Laminin
Rodentia
Plastics
Carbohydrates
Tunicamycin
Group Processes
Muscle Development
Growth
Cell culture
Lectins
Rats
Tumors
Melanoma
Glycoproteins
Adhesion
Experiments
Cells

Keywords

  • ConA
  • Glycosylation
  • Laminin
  • Lectins
  • Myoblasts

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Rodent myoblast interactions with laminin require cell surface glycoconjugates but not laminin glycosyl groups. / Kostrominova, Tatiana; Tanzer, M. L.

In: Journal of Cellular Biochemistry, Vol. 57, No. 1, 1995, p. 163-172.

Research output: Contribution to journalArticle

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