Role for IL-4 nonproducing NKT cells in CC-chemokine ligand 2-induced Th2 cell generation

Kazuhiko Fujita, Makiko Kobayashi, Randy R. Brutkiewicz, Toshiaki Hanafusa, David N. Herndon, Fujio Suzuki

Research output: Contribution to journalArticle

2 Scopus citations


NKT cells from C57Bl/6 mice are known to be the initial cellular source of IL-4 that acts as a trigger for Th2 cell differentiation. CC-chemokine ligand 2 (CCL2) has been described as an initial stimulator of IL-4 production by these cells; however, IL-4 was not produced by NKT cells from BALB/c mice even when Th2 cell responses were established in these mice. In this study, we found a new pathway for CCL2-associated Th2 cell generation in BALB/c mice. Splenic T cells from BALB/c mice produced IL-4 in response to CCL2 stimulation. However, IL-4 production was not seen in cultures of splenic T cells from CD1-/- mice (BALB/c origin), whereas, in the presence of CCL2, splenic T cells from CD1-/- mice produced IL-4 when NKT cells from wild-type mice were added. CCL2 induced IL-4 in cultures of NKT cells cocultured with naive T cells, but IL-4 was not produced by these cells cultured separately with CCL2. Interestingly, IL-4 was produced by naive T cells cocultured with NKT cells that were previously treated with CCL2 (CCL2-NKT cells). In addition, IL-4 was produced by naive T cells supplemented with a culture supernatant of CCL2-NKT cells. These results indicate that, through the production of a soluble factor(s) other than IL-4, NKT cells play a role in the CCL2-associated generation of Th2 cells.

Original languageEnglish (US)
Pages (from-to)44-50
Number of pages7
JournalImmunology and Cell Biology
Issue number1
StatePublished - Feb 1 2006



  • CC-chemokine ligand 2
  • NKT cell
  • Th2 response

ASJC Scopus subject areas

  • Immunology
  • Clinical Biochemistry
  • Cell Biology

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