Role of 4-1BB ligand in costimulation of T lymphocyte growth and its upregulation on M12 B lymphomas by cAMP

Mark A. DeBenedette, N. Randall Chu, Karen Pollok, José Hurtado, William F. Wade, Byoung S. Kwon, Tania H. Watts

Research output: Contribution to journalArticle

153 Citations (Scopus)

Abstract

K46J B lymphomas express a T cell costimulatory activity that is not inhibited by CTLA-4Ig, anti-B7-1, anti-B7-2, anti-intercellular adhesion molecule 1 or antibodies to heat stable antigen. In this paper we report that this costimulatory activity is mediated at least in part by 4-1BB ligand, a member of the tumor necrosis factor (TNF) gene family that binds to 4-1BB, a T cell activation antigen with homology to the TNF/nerve growth factor receptor family. A fusion protein between 4-1BB and alkaline phosphatase (4- 1BB-AP) blocks T cell activation by K46J lymphomas in both an antigen- specific system and with polyclonally (anti-CD3) activated T cells. 4-1BB-AP also blocks antigen presentation by normal spleen cells. When the antigen- presenting cells express B7 molecules as well as 4-1BB ligand, we find that B7 molecules and 4-1BB-AP both contribute to T cell activation. These data suggest that 4-1BB ligand plays an important role in costimulation of IL-2 production and proliferation by T cells. The B lymphoma M12 expresses low levels of 4-1BB-L but can be induced to express higher levels by treatment of the B cells with cAMP, which also induces B7-1 and B7-2 in these cells. Thus cAMP appears to coordinately induce several costimulatory molecules on B cells.

Original languageEnglish
Pages (from-to)985-992
Number of pages8
JournalJournal of Experimental Medicine
Volume181
Issue number3
DOIs
StatePublished - Mar 1 1995

Fingerprint

4-1BB Ligand
Lymphoma
Up-Regulation
T-Lymphocytes
B7 Antigens
Growth
B-Lymphocytes
Tumor Necrosis Factor-alpha
CD27 Antigens
Antigens
Nerve Growth Factor Receptor
Antigen Presentation
Antigen-Presenting Cells
Intercellular Adhesion Molecule-1
Interleukin-2
Alkaline Phosphatase
Spleen
Hot Temperature
Antibodies
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

Role of 4-1BB ligand in costimulation of T lymphocyte growth and its upregulation on M12 B lymphomas by cAMP. / DeBenedette, Mark A.; Chu, N. Randall; Pollok, Karen; Hurtado, José; Wade, William F.; Kwon, Byoung S.; Watts, Tania H.

In: Journal of Experimental Medicine, Vol. 181, No. 3, 01.03.1995, p. 985-992.

Research output: Contribution to journalArticle

DeBenedette, Mark A. ; Chu, N. Randall ; Pollok, Karen ; Hurtado, José ; Wade, William F. ; Kwon, Byoung S. ; Watts, Tania H. / Role of 4-1BB ligand in costimulation of T lymphocyte growth and its upregulation on M12 B lymphomas by cAMP. In: Journal of Experimental Medicine. 1995 ; Vol. 181, No. 3. pp. 985-992.
@article{36db7f1da0a9458f8d93e4f80659ce9d,
title = "Role of 4-1BB ligand in costimulation of T lymphocyte growth and its upregulation on M12 B lymphomas by cAMP",
abstract = "K46J B lymphomas express a T cell costimulatory activity that is not inhibited by CTLA-4Ig, anti-B7-1, anti-B7-2, anti-intercellular adhesion molecule 1 or antibodies to heat stable antigen. In this paper we report that this costimulatory activity is mediated at least in part by 4-1BB ligand, a member of the tumor necrosis factor (TNF) gene family that binds to 4-1BB, a T cell activation antigen with homology to the TNF/nerve growth factor receptor family. A fusion protein between 4-1BB and alkaline phosphatase (4- 1BB-AP) blocks T cell activation by K46J lymphomas in both an antigen- specific system and with polyclonally (anti-CD3) activated T cells. 4-1BB-AP also blocks antigen presentation by normal spleen cells. When the antigen- presenting cells express B7 molecules as well as 4-1BB ligand, we find that B7 molecules and 4-1BB-AP both contribute to T cell activation. These data suggest that 4-1BB ligand plays an important role in costimulation of IL-2 production and proliferation by T cells. The B lymphoma M12 expresses low levels of 4-1BB-L but can be induced to express higher levels by treatment of the B cells with cAMP, which also induces B7-1 and B7-2 in these cells. Thus cAMP appears to coordinately induce several costimulatory molecules on B cells.",
author = "DeBenedette, {Mark A.} and Chu, {N. Randall} and Karen Pollok and Jos{\'e} Hurtado and Wade, {William F.} and Kwon, {Byoung S.} and Watts, {Tania H.}",
year = "1995",
month = "3",
day = "1",
doi = "10.1084/jem.181.3.985",
language = "English",
volume = "181",
pages = "985--992",
journal = "Journal of Experimental Medicine",
issn = "0022-1007",
publisher = "Rockefeller University Press",
number = "3",

}

TY - JOUR

T1 - Role of 4-1BB ligand in costimulation of T lymphocyte growth and its upregulation on M12 B lymphomas by cAMP

AU - DeBenedette, Mark A.

AU - Chu, N. Randall

AU - Pollok, Karen

AU - Hurtado, José

AU - Wade, William F.

AU - Kwon, Byoung S.

AU - Watts, Tania H.

PY - 1995/3/1

Y1 - 1995/3/1

N2 - K46J B lymphomas express a T cell costimulatory activity that is not inhibited by CTLA-4Ig, anti-B7-1, anti-B7-2, anti-intercellular adhesion molecule 1 or antibodies to heat stable antigen. In this paper we report that this costimulatory activity is mediated at least in part by 4-1BB ligand, a member of the tumor necrosis factor (TNF) gene family that binds to 4-1BB, a T cell activation antigen with homology to the TNF/nerve growth factor receptor family. A fusion protein between 4-1BB and alkaline phosphatase (4- 1BB-AP) blocks T cell activation by K46J lymphomas in both an antigen- specific system and with polyclonally (anti-CD3) activated T cells. 4-1BB-AP also blocks antigen presentation by normal spleen cells. When the antigen- presenting cells express B7 molecules as well as 4-1BB ligand, we find that B7 molecules and 4-1BB-AP both contribute to T cell activation. These data suggest that 4-1BB ligand plays an important role in costimulation of IL-2 production and proliferation by T cells. The B lymphoma M12 expresses low levels of 4-1BB-L but can be induced to express higher levels by treatment of the B cells with cAMP, which also induces B7-1 and B7-2 in these cells. Thus cAMP appears to coordinately induce several costimulatory molecules on B cells.

AB - K46J B lymphomas express a T cell costimulatory activity that is not inhibited by CTLA-4Ig, anti-B7-1, anti-B7-2, anti-intercellular adhesion molecule 1 or antibodies to heat stable antigen. In this paper we report that this costimulatory activity is mediated at least in part by 4-1BB ligand, a member of the tumor necrosis factor (TNF) gene family that binds to 4-1BB, a T cell activation antigen with homology to the TNF/nerve growth factor receptor family. A fusion protein between 4-1BB and alkaline phosphatase (4- 1BB-AP) blocks T cell activation by K46J lymphomas in both an antigen- specific system and with polyclonally (anti-CD3) activated T cells. 4-1BB-AP also blocks antigen presentation by normal spleen cells. When the antigen- presenting cells express B7 molecules as well as 4-1BB ligand, we find that B7 molecules and 4-1BB-AP both contribute to T cell activation. These data suggest that 4-1BB ligand plays an important role in costimulation of IL-2 production and proliferation by T cells. The B lymphoma M12 expresses low levels of 4-1BB-L but can be induced to express higher levels by treatment of the B cells with cAMP, which also induces B7-1 and B7-2 in these cells. Thus cAMP appears to coordinately induce several costimulatory molecules on B cells.

UR - http://www.scopus.com/inward/record.url?scp=0028955074&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028955074&partnerID=8YFLogxK

U2 - 10.1084/jem.181.3.985

DO - 10.1084/jem.181.3.985

M3 - Article

C2 - 7532686

AN - SCOPUS:0028955074

VL - 181

SP - 985

EP - 992

JO - Journal of Experimental Medicine

JF - Journal of Experimental Medicine

SN - 0022-1007

IS - 3

ER -