Role of accessory cells in B cell activation. I. Macrophage presentation of TNP-Ficoll: Evidence for macrophage-B cell interaction

H. S. Boswell, S. O. Sharrow, A. Singer

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Abstract

The importance of cell interaction for thymic independent antigen responses has not been widely appreciated. The present report demonstrates, however, that macrophage-B cell interaction may be an important feature of B cell activation for the response to at least one polysaccharide thymic independent antigen, TNP-Ficoll. Experiments were performed demonstrating that a strict accessory cell requirement exists for the thymic independent response to soluble TNP-Ficoll, and that such accessory cells are both adherent and phagocytic, that is, macrophages. It was further demonstrated that macrophages could be pulsed with TNP-Ficoll and that these pulsed macrophages could activate B cells to respond, but only if the pulsed macrophages were viable. Thus, one function that macrophages can fulfill in responses to TNP-Ficoll is the specific function of antigen presentation. Such presentation of TNP-Ficoll by macrophages to B cells suggests that the antigen may not be activating B cells directly, and raises the possibility that the interaction of B cells and macrophages might be genetically restricted.

Original languageEnglish (US)
Pages (from-to)989-996
Number of pages8
JournalJournal of Immunology
Volume124
Issue number2
StatePublished - Jan 1 1980

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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