Role of ATP-sensitive potassium channels in the biphasic effects of morphine on pentylenetetrazole-induced seizure threshold in mice

Hamed Shafaroodi, Shahrzad Asadi, Hamed Sadeghipour, Mehdi Ghasemi, Farzad Ebrahimi, Sina Tavakoli, Amir Reza Hajrasouliha, Ahmad Reza Dehpour

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

Although several studies have indicated that the opioid receptor agonist morphine exerts biphasic effects on clonic seizure threshold, as yet little is known of the underlying mechanisms in this effect. In the present study, using the specific ATP-sensitive K+ (KATP) channel blocker glibenclamide and the specific KATP channel opener cromakalim, the possible involvement of KATP channels in the effects of morphine on pentylenetetrazole (PTZ)-induced seizure threshold in mice was investigated. Acute administration of lower doses of morphine (1, 3 and 7.5 mg/kg, i.p.) increased and higher doses of morphine (30 and 60 mg/kg, i.p.) decreased the PTZ-induced seizure threshold. Glibenclamide (2.5-5 mg/kg) increased the PTZ-induced seizure threshold. Non-effective dose of cromakalim (0.1 μg/kg) inhibited anticonvulsant effect of glibenclamide (5 mg/kg). Acute administration of non-effective dose of glibenclamide (1 mg/kg) interestingly inhibited both anticonvulsant and proconvulsant effects of morphine and this effect was significantly reversed by cromakalim (0.1 μg/kg). These results support the involvement of KATP channels in the modulation of seizure threshold by morphine.

Original languageEnglish (US)
Pages (from-to)63-69
Number of pages7
JournalEpilepsy Research
Volume75
Issue number1
DOIs
StatePublished - Jun 1 2007
Externally publishedYes

Keywords

  • ATP-sensitive potassium channel
  • Clonic seizure threshold
  • Mice
  • Morphine
  • Pentylentetrazole

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

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