Abstract
Although several studies have indicated that the opioid receptor agonist morphine exerts biphasic effects on clonic seizure threshold, as yet little is known of the underlying mechanisms in this effect. In the present study, using the specific ATP-sensitive K + (K ATP ) channel blocker glibenclamide and the specific K ATP channel opener cromakalim, the possible involvement of K ATP channels in the effects of morphine on pentylenetetrazole (PTZ)-induced seizure threshold in mice was investigated. Acute administration of lower doses of morphine (1, 3 and 7.5 mg/kg, i.p.) increased and higher doses of morphine (30 and 60 mg/kg, i.p.) decreased the PTZ-induced seizure threshold. Glibenclamide (2.5-5 mg/kg) increased the PTZ-induced seizure threshold. Non-effective dose of cromakalim (0.1 μg/kg) inhibited anticonvulsant effect of glibenclamide (5 mg/kg). Acute administration of non-effective dose of glibenclamide (1 mg/kg) interestingly inhibited both anticonvulsant and proconvulsant effects of morphine and this effect was significantly reversed by cromakalim (0.1 μg/kg). These results support the involvement of K ATP channels in the modulation of seizure threshold by morphine.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 63-69 |
| Number of pages | 7 |
| Journal | Epilepsy Research |
| Volume | 75 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jun 1 2007 |
| Externally published | Yes |
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Keywords
- ATP-sensitive potassium channel
- Clonic seizure threshold
- Mice
- Morphine
- Pentylentetrazole
ASJC Scopus subject areas
- Neurology
- Clinical Neurology
Cite this
Role of ATP-sensitive potassium channels in the biphasic effects of morphine on pentylenetetrazole-induced seizure threshold in mice. / Shafaroodi, Hamed; Asadi, Shahrzad; Sadeghipour, Hamed; Ghasemi, Mehdi; Ebrahimi, Farzad; Tavakoli, Sina; Hajrasouliha, Amir R.; Dehpour, Ahmad Reza.
In: Epilepsy Research, Vol. 75, No. 1, 01.06.2007, p. 63-69.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Role of ATP-sensitive potassium channels in the biphasic effects of morphine on pentylenetetrazole-induced seizure threshold in mice
AU - Shafaroodi, Hamed
AU - Asadi, Shahrzad
AU - Sadeghipour, Hamed
AU - Ghasemi, Mehdi
AU - Ebrahimi, Farzad
AU - Tavakoli, Sina
AU - Hajrasouliha, Amir R.
AU - Dehpour, Ahmad Reza
PY - 2007/6/1
Y1 - 2007/6/1
N2 - Although several studies have indicated that the opioid receptor agonist morphine exerts biphasic effects on clonic seizure threshold, as yet little is known of the underlying mechanisms in this effect. In the present study, using the specific ATP-sensitive K + (K ATP ) channel blocker glibenclamide and the specific K ATP channel opener cromakalim, the possible involvement of K ATP channels in the effects of morphine on pentylenetetrazole (PTZ)-induced seizure threshold in mice was investigated. Acute administration of lower doses of morphine (1, 3 and 7.5 mg/kg, i.p.) increased and higher doses of morphine (30 and 60 mg/kg, i.p.) decreased the PTZ-induced seizure threshold. Glibenclamide (2.5-5 mg/kg) increased the PTZ-induced seizure threshold. Non-effective dose of cromakalim (0.1 μg/kg) inhibited anticonvulsant effect of glibenclamide (5 mg/kg). Acute administration of non-effective dose of glibenclamide (1 mg/kg) interestingly inhibited both anticonvulsant and proconvulsant effects of morphine and this effect was significantly reversed by cromakalim (0.1 μg/kg). These results support the involvement of K ATP channels in the modulation of seizure threshold by morphine.
AB - Although several studies have indicated that the opioid receptor agonist morphine exerts biphasic effects on clonic seizure threshold, as yet little is known of the underlying mechanisms in this effect. In the present study, using the specific ATP-sensitive K + (K ATP ) channel blocker glibenclamide and the specific K ATP channel opener cromakalim, the possible involvement of K ATP channels in the effects of morphine on pentylenetetrazole (PTZ)-induced seizure threshold in mice was investigated. Acute administration of lower doses of morphine (1, 3 and 7.5 mg/kg, i.p.) increased and higher doses of morphine (30 and 60 mg/kg, i.p.) decreased the PTZ-induced seizure threshold. Glibenclamide (2.5-5 mg/kg) increased the PTZ-induced seizure threshold. Non-effective dose of cromakalim (0.1 μg/kg) inhibited anticonvulsant effect of glibenclamide (5 mg/kg). Acute administration of non-effective dose of glibenclamide (1 mg/kg) interestingly inhibited both anticonvulsant and proconvulsant effects of morphine and this effect was significantly reversed by cromakalim (0.1 μg/kg). These results support the involvement of K ATP channels in the modulation of seizure threshold by morphine.
KW - ATP-sensitive potassium channel
KW - Clonic seizure threshold
KW - Mice
KW - Morphine
KW - Pentylentetrazole
UR - http://www.scopus.com/inward/record.url?scp=34249794550&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34249794550&partnerID=8YFLogxK
U2 - 10.1016/j.eplepsyres.2007.04.005
DO - 10.1016/j.eplepsyres.2007.04.005
M3 - Article
VL - 75
SP - 63
EP - 69
JO - Epilepsy Research
JF - Epilepsy Research
SN - 0920-1211
IS - 1
ER -