Role of ATP-sensitive potassium channels in the biphasic effects of morphine on pentylenetetrazole-induced seizure threshold in mice

Hamed Shafaroodi, Shahrzad Asadi, Hamed Sadeghipour, Mehdi Ghasemi, Farzad Ebrahimi, Sina Tavakoli, Amir R. Hajrasouliha, Ahmad Reza Dehpour

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Although several studies have indicated that the opioid receptor agonist morphine exerts biphasic effects on clonic seizure threshold, as yet little is known of the underlying mechanisms in this effect. In the present study, using the specific ATP-sensitive K + (K ATP ) channel blocker glibenclamide and the specific K ATP channel opener cromakalim, the possible involvement of K ATP channels in the effects of morphine on pentylenetetrazole (PTZ)-induced seizure threshold in mice was investigated. Acute administration of lower doses of morphine (1, 3 and 7.5 mg/kg, i.p.) increased and higher doses of morphine (30 and 60 mg/kg, i.p.) decreased the PTZ-induced seizure threshold. Glibenclamide (2.5-5 mg/kg) increased the PTZ-induced seizure threshold. Non-effective dose of cromakalim (0.1 μg/kg) inhibited anticonvulsant effect of glibenclamide (5 mg/kg). Acute administration of non-effective dose of glibenclamide (1 mg/kg) interestingly inhibited both anticonvulsant and proconvulsant effects of morphine and this effect was significantly reversed by cromakalim (0.1 μg/kg). These results support the involvement of K ATP channels in the modulation of seizure threshold by morphine.

Original languageEnglish (US)
Pages (from-to)63-69
Number of pages7
JournalEpilepsy Research
Volume75
Issue number1
DOIs
StatePublished - Jun 1 2007
Externally publishedYes

Fingerprint

KATP Channels
Pentylenetetrazole
Morphine
Seizures
Glyburide
Cromakalim
Adenosine Triphosphate
Anticonvulsants
Opioid Receptors

Keywords

  • ATP-sensitive potassium channel
  • Clonic seizure threshold
  • Mice
  • Morphine
  • Pentylentetrazole

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

Cite this

Role of ATP-sensitive potassium channels in the biphasic effects of morphine on pentylenetetrazole-induced seizure threshold in mice. / Shafaroodi, Hamed; Asadi, Shahrzad; Sadeghipour, Hamed; Ghasemi, Mehdi; Ebrahimi, Farzad; Tavakoli, Sina; Hajrasouliha, Amir R.; Dehpour, Ahmad Reza.

In: Epilepsy Research, Vol. 75, No. 1, 01.06.2007, p. 63-69.

Research output: Contribution to journalArticle

Shafaroodi, Hamed ; Asadi, Shahrzad ; Sadeghipour, Hamed ; Ghasemi, Mehdi ; Ebrahimi, Farzad ; Tavakoli, Sina ; Hajrasouliha, Amir R. ; Dehpour, Ahmad Reza. / Role of ATP-sensitive potassium channels in the biphasic effects of morphine on pentylenetetrazole-induced seizure threshold in mice. In: Epilepsy Research. 2007 ; Vol. 75, No. 1. pp. 63-69.
@article{08e6f3ed336a48758d3f5ec891bcaf2d,
title = "Role of ATP-sensitive potassium channels in the biphasic effects of morphine on pentylenetetrazole-induced seizure threshold in mice",
abstract = "Although several studies have indicated that the opioid receptor agonist morphine exerts biphasic effects on clonic seizure threshold, as yet little is known of the underlying mechanisms in this effect. In the present study, using the specific ATP-sensitive K + (K ATP ) channel blocker glibenclamide and the specific K ATP channel opener cromakalim, the possible involvement of K ATP channels in the effects of morphine on pentylenetetrazole (PTZ)-induced seizure threshold in mice was investigated. Acute administration of lower doses of morphine (1, 3 and 7.5 mg/kg, i.p.) increased and higher doses of morphine (30 and 60 mg/kg, i.p.) decreased the PTZ-induced seizure threshold. Glibenclamide (2.5-5 mg/kg) increased the PTZ-induced seizure threshold. Non-effective dose of cromakalim (0.1 μg/kg) inhibited anticonvulsant effect of glibenclamide (5 mg/kg). Acute administration of non-effective dose of glibenclamide (1 mg/kg) interestingly inhibited both anticonvulsant and proconvulsant effects of morphine and this effect was significantly reversed by cromakalim (0.1 μg/kg). These results support the involvement of K ATP channels in the modulation of seizure threshold by morphine.",
keywords = "ATP-sensitive potassium channel, Clonic seizure threshold, Mice, Morphine, Pentylentetrazole",
author = "Hamed Shafaroodi and Shahrzad Asadi and Hamed Sadeghipour and Mehdi Ghasemi and Farzad Ebrahimi and Sina Tavakoli and Hajrasouliha, {Amir R.} and Dehpour, {Ahmad Reza}",
year = "2007",
month = "6",
day = "1",
doi = "10.1016/j.eplepsyres.2007.04.005",
language = "English (US)",
volume = "75",
pages = "63--69",
journal = "Epilepsy Research",
issn = "0920-1211",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Role of ATP-sensitive potassium channels in the biphasic effects of morphine on pentylenetetrazole-induced seizure threshold in mice

AU - Shafaroodi, Hamed

AU - Asadi, Shahrzad

AU - Sadeghipour, Hamed

AU - Ghasemi, Mehdi

AU - Ebrahimi, Farzad

AU - Tavakoli, Sina

AU - Hajrasouliha, Amir R.

AU - Dehpour, Ahmad Reza

PY - 2007/6/1

Y1 - 2007/6/1

N2 - Although several studies have indicated that the opioid receptor agonist morphine exerts biphasic effects on clonic seizure threshold, as yet little is known of the underlying mechanisms in this effect. In the present study, using the specific ATP-sensitive K + (K ATP ) channel blocker glibenclamide and the specific K ATP channel opener cromakalim, the possible involvement of K ATP channels in the effects of morphine on pentylenetetrazole (PTZ)-induced seizure threshold in mice was investigated. Acute administration of lower doses of morphine (1, 3 and 7.5 mg/kg, i.p.) increased and higher doses of morphine (30 and 60 mg/kg, i.p.) decreased the PTZ-induced seizure threshold. Glibenclamide (2.5-5 mg/kg) increased the PTZ-induced seizure threshold. Non-effective dose of cromakalim (0.1 μg/kg) inhibited anticonvulsant effect of glibenclamide (5 mg/kg). Acute administration of non-effective dose of glibenclamide (1 mg/kg) interestingly inhibited both anticonvulsant and proconvulsant effects of morphine and this effect was significantly reversed by cromakalim (0.1 μg/kg). These results support the involvement of K ATP channels in the modulation of seizure threshold by morphine.

AB - Although several studies have indicated that the opioid receptor agonist morphine exerts biphasic effects on clonic seizure threshold, as yet little is known of the underlying mechanisms in this effect. In the present study, using the specific ATP-sensitive K + (K ATP ) channel blocker glibenclamide and the specific K ATP channel opener cromakalim, the possible involvement of K ATP channels in the effects of morphine on pentylenetetrazole (PTZ)-induced seizure threshold in mice was investigated. Acute administration of lower doses of morphine (1, 3 and 7.5 mg/kg, i.p.) increased and higher doses of morphine (30 and 60 mg/kg, i.p.) decreased the PTZ-induced seizure threshold. Glibenclamide (2.5-5 mg/kg) increased the PTZ-induced seizure threshold. Non-effective dose of cromakalim (0.1 μg/kg) inhibited anticonvulsant effect of glibenclamide (5 mg/kg). Acute administration of non-effective dose of glibenclamide (1 mg/kg) interestingly inhibited both anticonvulsant and proconvulsant effects of morphine and this effect was significantly reversed by cromakalim (0.1 μg/kg). These results support the involvement of K ATP channels in the modulation of seizure threshold by morphine.

KW - ATP-sensitive potassium channel

KW - Clonic seizure threshold

KW - Mice

KW - Morphine

KW - Pentylentetrazole

UR - http://www.scopus.com/inward/record.url?scp=34249794550&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34249794550&partnerID=8YFLogxK

U2 - 10.1016/j.eplepsyres.2007.04.005

DO - 10.1016/j.eplepsyres.2007.04.005

M3 - Article

C2 - 17517498

AN - SCOPUS:34249794550

VL - 75

SP - 63

EP - 69

JO - Epilepsy Research

JF - Epilepsy Research

SN - 0920-1211

IS - 1

ER -