Role of calcium in metabolic signaling between cardiac sarcoplasmic reticulum and mitochondria in vitro

Robert S. Balaban, Salil Bose, Stephanie A. French, Paul Territo

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The role of Ca2+ as a cytosolic signaling molecule between porcine cardiac sarcoplasmic reticulum (SR) ATPase and mitochondrial ATP production was evaluated in vitro. The Ca2+ sensitivity of these processes was determined individually and in a reconstituted system with SR and mitochondria in a 0.5:1 protein-to-cytochrome aa3 ratio. The half-maximal concentration (K1/2) of SR ATPase was 335 nM Ca2+. The ATP synthesis dependence was similar with a K1/2 of 243 nM for dehydrogenases and 114 nM for overall ATP production. In the reconstituted system, Ca2+ increased thapsigargin-sensitive ATP production (maximum ∼5-fold) with minimal changes in mitochondrial reduced nicotinamide adenine dinucleotide (NADH). NADH concentration remained stable despite graded increases in NADH turnover induced over a wide range of Ca2+ concentrations (0 to ∼500 nM). These data are consistent with a balanced activation of SR ATPase and mitochondrial ATP synthesis by Ca2+ that contributes to a homeostasis of energy metabolism metabolites. It is suggested that this balanced activation by cytosolic Ca2+ is partially responsible for the minimal alteration in energy metabolism intermediates that occurs with changes in cardiac workload in vivo.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume284
Issue number2 53-2
StatePublished - Feb 1 2003
Externally publishedYes

Fingerprint

Mitochondria
Sarcoplasmic Reticulum
Adenosine Triphosphate
NAD
Calcium
Adenosine Triphosphatases
Energy Metabolism
Chemical activation
Thapsigargin
Electron Transport Complex IV
Metabolites
Workload
Oxidoreductases
Homeostasis
Swine
In Vitro Techniques
Molecules
Proteins

Keywords

  • Adenosine 5′-triphosphate
  • Calcium-adenosinetriphosphatase
  • Energy metabolism
  • Porcine heart
  • Reduced nicotinamide adenine dinucleotide

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Role of calcium in metabolic signaling between cardiac sarcoplasmic reticulum and mitochondria in vitro. / Balaban, Robert S.; Bose, Salil; French, Stephanie A.; Territo, Paul.

In: American Journal of Physiology - Cell Physiology, Vol. 284, No. 2 53-2, 01.02.2003.

Research output: Contribution to journalArticle

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