Role of cell-cycle in regulating neuroepithelial cell shape during bending of the chick neural plate

Jodi Smith, Gary C. Schoenwolf

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Neuroepithelial cells transform from spindle-shaped to wedge-shaped within the median and paired dorsolateral hinge points of the bending neural plate, but the mechanisms underlying these localized changes are unclear. This study was designed to evaluate further the hypothesis that localized "wedging" of neuroepithelial cells during bending involves basal cellular expansion resulting from alteration of the cell-cycle. Neurulating chick embryos were treated with tritiated thymidine, and transverse sections through the midbrain were examined autoradiographically. Parameters of the cell-cycle as well as nuclear position and size were assessed in the median hinge point, which contains predominantly wedge-shaped cells, and in adjacent lateral areas of the neural plate, which contain predominantly spindle-shaped cells. Both the DNA-synthetic phase and non-DNA synthetic portion of the cell-cycle were significantly longer in the median hinge point than in lateral neuroepithelial areas, some nuclei in both regions were located basally during these phases, and virtually all basal nuclei in the median hinge point were large. Additionally, the mitotic phase was significantly shorter in the median hinge point than in lateral areas. We present a model to explain how alteration of the cell-cycle in the median hinge point could generate wedging of cells in this region.

Original languageEnglish (US)
Pages (from-to)491-500
Number of pages10
JournalCell and Tissue Research
Volume252
Issue number3
DOIs
StatePublished - Jun 1988
Externally publishedYes

Fingerprint

Neuroepithelial Cells
Neural Plate
Cell Shape
Hinges
Cell Cycle
Cells
Artificial Cells
Chick Embryo
Mesencephalon
Basal Ganglia
Thymidine
DNA

Keywords

  • Autoradiography
  • Cell shape
  • Cell-cycle
  • Chick
  • Neural plate
  • Neuroepithelium
  • Neurulation
  • Tritiated thymidine

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

Role of cell-cycle in regulating neuroepithelial cell shape during bending of the chick neural plate. / Smith, Jodi; Schoenwolf, Gary C.

In: Cell and Tissue Research, Vol. 252, No. 3, 06.1988, p. 491-500.

Research output: Contribution to journalArticle

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