Role of lipooligosaccharides in experimental dermal lesions caused by Haemophilus ducreyi

A. A. Campagnari, L. M. Wild, G. E. Griffiths, R. J. Karalus, M. A. Wirth, Stanley Spinola

Research output: Contribution to journalArticle

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Abstract

The mouse and rabbit intradermal injection models have been used to define factors that may be important in Haemophilus ducreyi pathogenesis. We used H. ducreyi strains with diverse geographic origins and phenotypic characteristics to evaluate the experimental models. Injection of live and heat-killed bacteria caused skin abscesses in both models. Semiquantitative cultures of skin injected with live bacteria showed that H. ducreyi failed to replicate in animal tissue. These data suggested that the experimental lesions were caused by a heat-stable substance such as lipooligosaccharide (LOS). In mice, injection of H. ducreyi and Haemophilus influenzae LOS and Escherichia coli lipopolysaccharide caused mild to moderate inflammation. In rabbits, injection of H. ducreyi LOS caused intradermal abscesses that were histologically similar to those caused by live and heat-killed bacteria. H. ducreyi and Neisseria gonorrhoeae LOS caused significantly larger lesions than equivalent amounts of H. influenzae LOS and E. coli lipopolysaccharide in the rabbit model. We conclude that the intradermal injection models are not valid models to study the growth of H. ducreyi in vivo. However, these data indicate that H. ducreyi LOS may play an important role in the pathogenesis of chancroid and that the rabbit model should be useful in studying H. ducreyi LOS toxicity at the cellular level.

Original languageEnglish (US)
Pages (from-to)2601-2608
Number of pages8
JournalInfection and Immunity
Volume59
Issue number8
StatePublished - 1991
Externally publishedYes

Fingerprint

Haemophilus ducreyi
Skin
Rabbits
Intradermal Injections
Hot Temperature
Haemophilus influenzae
Bacteria
Abscess
Injections
Lipopolysaccharides
Chancroid
Escherichia coli
lipid-linked oligosaccharides
Neisseria gonorrhoeae
Theoretical Models

ASJC Scopus subject areas

  • Immunology

Cite this

Campagnari, A. A., Wild, L. M., Griffiths, G. E., Karalus, R. J., Wirth, M. A., & Spinola, S. (1991). Role of lipooligosaccharides in experimental dermal lesions caused by Haemophilus ducreyi. Infection and Immunity, 59(8), 2601-2608.

Role of lipooligosaccharides in experimental dermal lesions caused by Haemophilus ducreyi. / Campagnari, A. A.; Wild, L. M.; Griffiths, G. E.; Karalus, R. J.; Wirth, M. A.; Spinola, Stanley.

In: Infection and Immunity, Vol. 59, No. 8, 1991, p. 2601-2608.

Research output: Contribution to journalArticle

Campagnari, AA, Wild, LM, Griffiths, GE, Karalus, RJ, Wirth, MA & Spinola, S 1991, 'Role of lipooligosaccharides in experimental dermal lesions caused by Haemophilus ducreyi', Infection and Immunity, vol. 59, no. 8, pp. 2601-2608.
Campagnari AA, Wild LM, Griffiths GE, Karalus RJ, Wirth MA, Spinola S. Role of lipooligosaccharides in experimental dermal lesions caused by Haemophilus ducreyi. Infection and Immunity. 1991;59(8):2601-2608.
Campagnari, A. A. ; Wild, L. M. ; Griffiths, G. E. ; Karalus, R. J. ; Wirth, M. A. ; Spinola, Stanley. / Role of lipooligosaccharides in experimental dermal lesions caused by Haemophilus ducreyi. In: Infection and Immunity. 1991 ; Vol. 59, No. 8. pp. 2601-2608.
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