Role of macrophage-colony stimulating factor and osteoclast differentiation factor in osteoclastogenesis of bone marrow derived stem cells

Mohamed Bayoumy, Uma Sankar, Natarajan Muthusamy

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Macrophage colony stimulating factor (M-CSF) and osteoclast differentiation factor (ODF) regulate osteoclastogenesis in vivo. Regulation of osteoclast development in vitro by these cytokines has been reported in the present study. Simultaneous addition of ODF and M-CSF during initiation of bone marrow culture inhibited osteoclastogenesis. However, delayed addition of ODF (three days after initiation of the culture) resulted in dramatic increase in phenotypically and functionally mature osteoclast cells. Delayed addition of ODF beyond day three decreased osteoclastogenesis. Further, removal of M-CSF as early as day three inhibited ODF-induced osteoclastogenesis. These studies provided evidence for the importance of co-ordinated regulation of osteoclastogenesis by M-CSF and ODF.

Original languageEnglish (US)
Pages (from-to)995-1000
Number of pages6
JournalIndian Journal of Experimental Biology
Issue number9
StatePublished - Sep 1 2002


ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Cell Biology

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