Role of nucleotide pyrophosphatase in enflurane-induced reduction of UDP-glucuronic acid concentration in mouse liver

John B. Watkins, Mark A. Pierce

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Because hepatic UDP-glucuronic acid levels decrease upon exposure to volatile anesthetics, the present study was designed to determine the mechanism by which enflurane decreases UDP-glucuronic acid in mice by measuring the concentrations of intermediates and the activities of enzymes in the UDP-glucuronic acid pathway. UDP-glucuronic acid concentrations were decreased by 40% in both male and female mice after 10 min of enflurane-induced narcosis. Concentration of UDP-glucose and the activities of diethylstilbestrol UDP-glucuronosyltransferase and UDP-glucose dehydrogenase were not affected by enflurane treatment. In contrast, nucleotide pyrophophatase activity was increased approximately 50% in both sexes. Thus, the decrease in hepatic UDP-glucuronic acid upon exposure of mice to enflurane is probably due to increased degradation by nucleotide pyrophosphatase.

Original languageEnglish (US)
Pages (from-to)378-383
Number of pages6
JournalToxicology and Applied Pharmacology
Volume102
Issue number2
DOIs
StatePublished - Feb 1990

Fingerprint

nucleotide pyrophosphatase
Uridine Diphosphate Glucuronic Acid
Enflurane
Liver
Uridine Diphosphate Glucose Dehydrogenase
Uridine Diphosphate Glucose
Stupor
Glucuronosyltransferase
Diethylstilbestrol
Anesthetics
Nucleotides
Degradation
Enzymes

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology

Cite this

Role of nucleotide pyrophosphatase in enflurane-induced reduction of UDP-glucuronic acid concentration in mouse liver. / Watkins, John B.; Pierce, Mark A.

In: Toxicology and Applied Pharmacology, Vol. 102, No. 2, 02.1990, p. 378-383.

Research output: Contribution to journalArticle

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abstract = "Because hepatic UDP-glucuronic acid levels decrease upon exposure to volatile anesthetics, the present study was designed to determine the mechanism by which enflurane decreases UDP-glucuronic acid in mice by measuring the concentrations of intermediates and the activities of enzymes in the UDP-glucuronic acid pathway. UDP-glucuronic acid concentrations were decreased by 40{\%} in both male and female mice after 10 min of enflurane-induced narcosis. Concentration of UDP-glucose and the activities of diethylstilbestrol UDP-glucuronosyltransferase and UDP-glucose dehydrogenase were not affected by enflurane treatment. In contrast, nucleotide pyrophophatase activity was increased approximately 50{\%} in both sexes. Thus, the decrease in hepatic UDP-glucuronic acid upon exposure of mice to enflurane is probably due to increased degradation by nucleotide pyrophosphatase.",
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