Role of phagocyte oxidase in UVA-induced oxidative stress and apoptosis in keratinocytes

Yu Ying He, Jian Li Huang, Michelle L. Block, Jau Shyong Hong, Colin F. Chignell

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Chronic exposure to ultraviolet radiation including ultraviolet A (315-400 nm) (UVA) may cause photocarcinogenesis and photoaging. The UVA-induced production of reactive oxygen species (ROS) and the resultant oxidative stress exposure play an important role in these biological processes. Here we have investigated the role of phagocyte oxidase (PHOX, gp91phox) in the production of ROS, redox status change, and apoptosis after UVA exposure by using gp91phox-deficient (gp91phox-/-) primary keratinocytes. UVA radiation resulted in increased ROS production and oxidation of reduced glutathione (GSH) to its oxidized form (GSSG). The presence of diphenylene iodonium (DPI) inhibited ROS production by UVA. In comparison with wild-type cells, gp91 phox-/- cells produced slightly less ROS and GSH oxidation. UVA radiation induced apoptosis in wild-type keratinocytes as detected by phosphatidylserine (PS) translocation, caspase activation, and DNA fragmentation. As compared with wild-type cells, UVA induced less PS translocation in gp91phox-deficient cells. No difference, however, was observed in caspase activation and DNA fragmentation after UVA exposure in wild-type and gp91phox-/- cells. These findings suggest that gp91phox plays a limited role in the UVA-induced ROS production, oxidative stress, and therefore the PS translocation, but has no effect on UVA-induced caspase activation and DNA fragmentation during apoptosis.

Original languageEnglish (US)
Pages (from-to)560-566
Number of pages7
JournalJournal of Investigative Dermatology
Volume125
Issue number3
DOIs
StatePublished - Sep 1 2005

Fingerprint

Oxidative stress
Phagocytes
Keratinocytes
Reactive Oxygen Species
Oxidoreductases
Oxidative Stress
Apoptosis
Phosphatidylserines
DNA Fragmentation
Caspases
Chemical activation
Radiation
DNA
Biological Phenomena
Oxidation
Glutathione Disulfide
Ultraviolet radiation
Oxidation-Reduction
Glutathione

Keywords

  • Apoptosis
  • Keratinocytes
  • NADPH oxidase
  • Reactive oxygen species
  • UVA
  • gp91

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

Cite this

Role of phagocyte oxidase in UVA-induced oxidative stress and apoptosis in keratinocytes. / He, Yu Ying; Huang, Jian Li; Block, Michelle L.; Hong, Jau Shyong; Chignell, Colin F.

In: Journal of Investigative Dermatology, Vol. 125, No. 3, 01.09.2005, p. 560-566.

Research output: Contribution to journalArticle

He, Yu Ying ; Huang, Jian Li ; Block, Michelle L. ; Hong, Jau Shyong ; Chignell, Colin F. / Role of phagocyte oxidase in UVA-induced oxidative stress and apoptosis in keratinocytes. In: Journal of Investigative Dermatology. 2005 ; Vol. 125, No. 3. pp. 560-566.
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