Role of protein kinase Cα in regulation of [Ca2+] I and force in human myometrium

Victor P. Fomin, Andris Kronbergs, Susan Gunst, Dale Tang, Vladimir Simirskii, Matthew Hoffman, Randall L. Duncan

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Recent findings implicate protein kinase C in regulation of contraction of uterine muscle (myometrium). However, the role of protein kinase C isoforms in myometrial contraction remains uncertain. Therefore, this study examined protein kinase Cα's role in regulation of contraction and intracellular calcium concentration ([Ca2+]I) of myometrium from term pregnant women. The authors demonstrated that protein kinase Cα inhibitor Go6976 decreased the amplitude of potassium chloride-induced myometrial contractions in a time-dependent manner. The treatment of the myometrial strips with protein kinase Cα-specific antisense oligodeoxynucleotides decreased the potassium chloride-induced contraction and [Ca2+]I response to 39.3% + 6.8% and 50.0% + 3.3%, respectively, compared to control. The sense oligonucleotides treatment did not significantly change the potassium chloride responses (89.8% + 6.8% and 93.9% + 4.5% of the control for the contraction and [Ca2+]I, respectively). These data, coupled with the observation that protein kinase Cα levels are elevated in the pregnant myometrium, suggest the involvement of protein kinase Cα in regulation of human uterine contraction.

Original languageEnglish
Pages (from-to)71-79
Number of pages9
JournalReproductive Sciences
Volume16
Issue number1
DOIs
StatePublished - Jan 2009

Fingerprint

Myometrium
Protein Kinase C
Uterine Contraction
Potassium Chloride
Protein C Inhibitor
Oligodeoxyribonucleotides
Protein Kinase Inhibitors
Oligonucleotides
Protein Kinases
Pregnant Women
Protein Isoforms
Calcium
Therapeutics

Keywords

  • Human myometrium
  • Intracellular calcium
  • Pregnancy
  • Protein kinase C
  • Uterine muscle

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Fomin, V. P., Kronbergs, A., Gunst, S., Tang, D., Simirskii, V., Hoffman, M., & Duncan, R. L. (2009). Role of protein kinase Cα in regulation of [Ca2+] I and force in human myometrium. Reproductive Sciences, 16(1), 71-79. https://doi.org/10.1177/1933719108324892

Role of protein kinase Cα in regulation of [Ca2+] I and force in human myometrium. / Fomin, Victor P.; Kronbergs, Andris; Gunst, Susan; Tang, Dale; Simirskii, Vladimir; Hoffman, Matthew; Duncan, Randall L.

In: Reproductive Sciences, Vol. 16, No. 1, 01.2009, p. 71-79.

Research output: Contribution to journalArticle

Fomin, VP, Kronbergs, A, Gunst, S, Tang, D, Simirskii, V, Hoffman, M & Duncan, RL 2009, 'Role of protein kinase Cα in regulation of [Ca2+] I and force in human myometrium', Reproductive Sciences, vol. 16, no. 1, pp. 71-79. https://doi.org/10.1177/1933719108324892
Fomin, Victor P. ; Kronbergs, Andris ; Gunst, Susan ; Tang, Dale ; Simirskii, Vladimir ; Hoffman, Matthew ; Duncan, Randall L. / Role of protein kinase Cα in regulation of [Ca2+] I and force in human myometrium. In: Reproductive Sciences. 2009 ; Vol. 16, No. 1. pp. 71-79.
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