Role of protein kinase Cα in regulation of [Ca2+] I and force in human myometrium

Victor P. Fomin, Andris Kronbergs, Susan Gunst, Dale Tang, Vladimir Simirskii, Matthew Hoffman, Randall L. Duncan

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Abstract

Recent findings implicate protein kinase C in regulation of contraction of uterine muscle (myometrium). However, the role of protein kinase C isoforms in myometrial contraction remains uncertain. Therefore, this study examined protein kinase Cα's role in regulation of contraction and intracellular calcium concentration ([Ca2+]I) of myometrium from term pregnant women. The authors demonstrated that protein kinase Cα inhibitor Go6976 decreased the amplitude of potassium chloride-induced myometrial contractions in a time-dependent manner. The treatment of the myometrial strips with protein kinase Cα-specific antisense oligodeoxynucleotides decreased the potassium chloride-induced contraction and [Ca2+]I response to 39.3% + 6.8% and 50.0% + 3.3%, respectively, compared to control. The sense oligonucleotides treatment did not significantly change the potassium chloride responses (89.8% + 6.8% and 93.9% + 4.5% of the control for the contraction and [Ca2+]I, respectively). These data, coupled with the observation that protein kinase Cα levels are elevated in the pregnant myometrium, suggest the involvement of protein kinase Cα in regulation of human uterine contraction.

Original languageEnglish (US)
Pages (from-to)71-79
Number of pages9
JournalReproductive Sciences
Volume16
Issue number1
DOIs
StatePublished - Jan 1 2009

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Keywords

  • Human myometrium
  • Intracellular calcium
  • Pregnancy
  • Protein kinase C
  • Uterine muscle

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Fomin, V. P., Kronbergs, A., Gunst, S., Tang, D., Simirskii, V., Hoffman, M., & Duncan, R. L. (2009). Role of protein kinase Cα in regulation of [Ca2+] I and force in human myometrium. Reproductive Sciences, 16(1), 71-79. https://doi.org/10.1177/1933719108324892