Role of semaphorin-1a in the developing visual system of the disease vector mosquito Aedes aegypti

Keshava Mysore, Ellen Flannery, Matthew T. Leming, Michael Tomchaney, Lucy Shi, Longhua Sun, Joseph E. O'Tousa, David W. Severson, Molly Scheel

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: Despite the devastating impact of mosquito-borne illnesses on human health, very little is known about mosquito developmental biology, including development of the mosquito visual system. Mosquitoes possess functional adult compound eyes as larvae, a trait that makes them an interesting model in which to study comparative developmental genetics. Here, we functionally characterize visual system development in the dengue and yellow fever vector mosquito Aedes aegypti, in which we use chitosan/siRNA nanoparticles to target the axon guidance gene semaphorin-1a (sema1a). Results: Immunohistochemical analyses revealed the progression of visual sensory neuron targeting that results in generation of the retinotopic map in the mosquito optic lobe. Loss of sema1a function led to optic lobe phenotypes, including defective targeting of visual sensory neurons and failed formation of the retinotopic map. These sema1a knockdown phenotypes correlated with behavioral defects in larval photoavoidance. Conclusions: The results of this investigation indicate that Sema1a is required for optic lobe development in A. aegypti and highlight the behavioral importance of a functioning visual system in preadult mosquitoes.

Original languageEnglish
Pages (from-to)1457-1469
Number of pages13
JournalDevelopmental Dynamics
Volume243
Issue number11
DOIs
StatePublished - Nov 1 2014

Fingerprint

Semaphorins
Disease Vectors
Aedes
Culicidae
Sensory Receptor Cells
Phenotype
Yellow Fever
Developmental Biology
Dengue
Chitosan
Nanoparticles
Small Interfering RNA
Larva
Health
Genes

Keywords

  • Aedes aegypti
  • Development
  • Eye
  • Mosquito
  • Nanoparticle
  • Optic lobe
  • Semaphorin
  • Sensory system
  • SiRNA
  • Targeting
  • Vision

ASJC Scopus subject areas

  • Developmental Biology
  • Medicine(all)

Cite this

Role of semaphorin-1a in the developing visual system of the disease vector mosquito Aedes aegypti. / Mysore, Keshava; Flannery, Ellen; Leming, Matthew T.; Tomchaney, Michael; Shi, Lucy; Sun, Longhua; O'Tousa, Joseph E.; Severson, David W.; Scheel, Molly.

In: Developmental Dynamics, Vol. 243, No. 11, 01.11.2014, p. 1457-1469.

Research output: Contribution to journalArticle

Mysore, K, Flannery, E, Leming, MT, Tomchaney, M, Shi, L, Sun, L, O'Tousa, JE, Severson, DW & Scheel, M 2014, 'Role of semaphorin-1a in the developing visual system of the disease vector mosquito Aedes aegypti', Developmental Dynamics, vol. 243, no. 11, pp. 1457-1469. https://doi.org/10.1002/dvdy.24168
Mysore, Keshava ; Flannery, Ellen ; Leming, Matthew T. ; Tomchaney, Michael ; Shi, Lucy ; Sun, Longhua ; O'Tousa, Joseph E. ; Severson, David W. ; Scheel, Molly. / Role of semaphorin-1a in the developing visual system of the disease vector mosquito Aedes aegypti. In: Developmental Dynamics. 2014 ; Vol. 243, No. 11. pp. 1457-1469.
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