Role of sortase in Streptococcus mutans under the effect of nicotine

Ming Yun Li, Rui Jie Huang, Xue Dong Zhou, Richard Gregory

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Streptococcus mutans is a common Gram-positive bacterium and plays a significant role in dental caries. Tobacco and/or nicotine have documented effects on S. mutans growth and colonization. Sortase A is used by many Gram-positive bacteria, including S. mutans, to facilitate the insertion of certain cell surface proteins, containing an LPXTGX motif such as antigen I/II. This study examined the effect of nicotine on the function of sortase A to control the physiology and growth of S. mutans using wild-type S. mutans NG8, and its isogenic sortase-defective and -complemented strains. Briefly, the strains were treated with increasing amounts of nicotine in planktonic growth, biofilm metabolism, and sucrose-induced and saliva-induced antigen I/II-dependent biofilm formation assays. The strains exhibited no significant differences with different concentrations of nicotine in planktonic growth assays. However, they had significantly increased (P≤0.05) biofilm metabolic activity (2- to 3-fold increase) as the concentration of nicotine increased. Furthermore, the sortase-defective strain was more sensitive metabolically to nicotine than the wild-type or sortase-complemented strains. All strains had significantly increased sucrose-induced biofilm formation (2- to 3-fold increase) as a result of increasing concentrations of nicotine. However, the sortase-defective strain was not able to make as much sucrose- and saliva-induced biofilm as the wild-type NG8 did with increasing nicotine concentrations. These results indicated that nicotine increased metabolic activity and sucrose-induced biofilm formation. The saliva-induced biofilm formation assay and qPCR data suggested that antigen I/II was upregulated with nicotine but biofilm was not able to be formed as much as wild-type NG8 without functional sortase A.

Original languageEnglish
Pages (from-to)206-211
Number of pages6
JournalInternational journal of oral science
Volume5
Issue number4
DOIs
StatePublished - Dec 2013

Fingerprint

Streptococcus mutans
Nicotine
Biofilms
Sucrose
Saliva
Gram-Positive Bacteria
Growth
Dental Caries
Tobacco
Membrane Proteins

Keywords

  • dental caries
  • metabolism
  • nicotine
  • sortase A
  • Streptococcus mutans

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Role of sortase in Streptococcus mutans under the effect of nicotine. / Li, Ming Yun; Huang, Rui Jie; Zhou, Xue Dong; Gregory, Richard.

In: International journal of oral science, Vol. 5, No. 4, 12.2013, p. 206-211.

Research output: Contribution to journalArticle

Li, Ming Yun ; Huang, Rui Jie ; Zhou, Xue Dong ; Gregory, Richard. / Role of sortase in Streptococcus mutans under the effect of nicotine. In: International journal of oral science. 2013 ; Vol. 5, No. 4. pp. 206-211.
@article{ed611aa07a4b4be5bf8f7dfa7e20eb15,
title = "Role of sortase in Streptococcus mutans under the effect of nicotine",
abstract = "Streptococcus mutans is a common Gram-positive bacterium and plays a significant role in dental caries. Tobacco and/or nicotine have documented effects on S. mutans growth and colonization. Sortase A is used by many Gram-positive bacteria, including S. mutans, to facilitate the insertion of certain cell surface proteins, containing an LPXTGX motif such as antigen I/II. This study examined the effect of nicotine on the function of sortase A to control the physiology and growth of S. mutans using wild-type S. mutans NG8, and its isogenic sortase-defective and -complemented strains. Briefly, the strains were treated with increasing amounts of nicotine in planktonic growth, biofilm metabolism, and sucrose-induced and saliva-induced antigen I/II-dependent biofilm formation assays. The strains exhibited no significant differences with different concentrations of nicotine in planktonic growth assays. However, they had significantly increased (P≤0.05) biofilm metabolic activity (2- to 3-fold increase) as the concentration of nicotine increased. Furthermore, the sortase-defective strain was more sensitive metabolically to nicotine than the wild-type or sortase-complemented strains. All strains had significantly increased sucrose-induced biofilm formation (2- to 3-fold increase) as a result of increasing concentrations of nicotine. However, the sortase-defective strain was not able to make as much sucrose- and saliva-induced biofilm as the wild-type NG8 did with increasing nicotine concentrations. These results indicated that nicotine increased metabolic activity and sucrose-induced biofilm formation. The saliva-induced biofilm formation assay and qPCR data suggested that antigen I/II was upregulated with nicotine but biofilm was not able to be formed as much as wild-type NG8 without functional sortase A.",
keywords = "dental caries, metabolism, nicotine, sortase A, Streptococcus mutans",
author = "Li, {Ming Yun} and Huang, {Rui Jie} and Zhou, {Xue Dong} and Richard Gregory",
year = "2013",
month = "12",
doi = "10.1038/ijos.2013.86",
language = "English",
volume = "5",
pages = "206--211",
journal = "International journal of oral science",
issn = "1674-2818",
publisher = "Sichuan University Press",
number = "4",

}

TY - JOUR

T1 - Role of sortase in Streptococcus mutans under the effect of nicotine

AU - Li, Ming Yun

AU - Huang, Rui Jie

AU - Zhou, Xue Dong

AU - Gregory, Richard

PY - 2013/12

Y1 - 2013/12

N2 - Streptococcus mutans is a common Gram-positive bacterium and plays a significant role in dental caries. Tobacco and/or nicotine have documented effects on S. mutans growth and colonization. Sortase A is used by many Gram-positive bacteria, including S. mutans, to facilitate the insertion of certain cell surface proteins, containing an LPXTGX motif such as antigen I/II. This study examined the effect of nicotine on the function of sortase A to control the physiology and growth of S. mutans using wild-type S. mutans NG8, and its isogenic sortase-defective and -complemented strains. Briefly, the strains were treated with increasing amounts of nicotine in planktonic growth, biofilm metabolism, and sucrose-induced and saliva-induced antigen I/II-dependent biofilm formation assays. The strains exhibited no significant differences with different concentrations of nicotine in planktonic growth assays. However, they had significantly increased (P≤0.05) biofilm metabolic activity (2- to 3-fold increase) as the concentration of nicotine increased. Furthermore, the sortase-defective strain was more sensitive metabolically to nicotine than the wild-type or sortase-complemented strains. All strains had significantly increased sucrose-induced biofilm formation (2- to 3-fold increase) as a result of increasing concentrations of nicotine. However, the sortase-defective strain was not able to make as much sucrose- and saliva-induced biofilm as the wild-type NG8 did with increasing nicotine concentrations. These results indicated that nicotine increased metabolic activity and sucrose-induced biofilm formation. The saliva-induced biofilm formation assay and qPCR data suggested that antigen I/II was upregulated with nicotine but biofilm was not able to be formed as much as wild-type NG8 without functional sortase A.

AB - Streptococcus mutans is a common Gram-positive bacterium and plays a significant role in dental caries. Tobacco and/or nicotine have documented effects on S. mutans growth and colonization. Sortase A is used by many Gram-positive bacteria, including S. mutans, to facilitate the insertion of certain cell surface proteins, containing an LPXTGX motif such as antigen I/II. This study examined the effect of nicotine on the function of sortase A to control the physiology and growth of S. mutans using wild-type S. mutans NG8, and its isogenic sortase-defective and -complemented strains. Briefly, the strains were treated with increasing amounts of nicotine in planktonic growth, biofilm metabolism, and sucrose-induced and saliva-induced antigen I/II-dependent biofilm formation assays. The strains exhibited no significant differences with different concentrations of nicotine in planktonic growth assays. However, they had significantly increased (P≤0.05) biofilm metabolic activity (2- to 3-fold increase) as the concentration of nicotine increased. Furthermore, the sortase-defective strain was more sensitive metabolically to nicotine than the wild-type or sortase-complemented strains. All strains had significantly increased sucrose-induced biofilm formation (2- to 3-fold increase) as a result of increasing concentrations of nicotine. However, the sortase-defective strain was not able to make as much sucrose- and saliva-induced biofilm as the wild-type NG8 did with increasing nicotine concentrations. These results indicated that nicotine increased metabolic activity and sucrose-induced biofilm formation. The saliva-induced biofilm formation assay and qPCR data suggested that antigen I/II was upregulated with nicotine but biofilm was not able to be formed as much as wild-type NG8 without functional sortase A.

KW - dental caries

KW - metabolism

KW - nicotine

KW - sortase A

KW - Streptococcus mutans

UR - http://www.scopus.com/inward/record.url?scp=84889765297&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84889765297&partnerID=8YFLogxK

U2 - 10.1038/ijos.2013.86

DO - 10.1038/ijos.2013.86

M3 - Article

C2 - 24136674

AN - SCOPUS:84889765297

VL - 5

SP - 206

EP - 211

JO - International journal of oral science

JF - International journal of oral science

SN - 1674-2818

IS - 4

ER -