Role of STAT3 in liver regeneration

Survival, DNA synthesis, inflammatory reaction and liver mass recovery

Akira Moh, Yoshiki Iwamoto, Gui Xuan Chai, Samual Shao Min Zhang, Arihiro Kano, Derek D. Yang, Wenjun Zhang, Jun Wang, Joerg J. Jacoby, Bin Gao, Richard A. Flavell, Xin Yuan Fu

Research output: Contribution to journalArticle

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Abstract

The hepatoprotective effect of interleukin-6 (IL-6)/signal transducer and activator of transcription 3 (STAT3) has been well documented. However, reports on the role of IL-6/STAT3 in liver regeneration are conflicting probably due to the fact that the model of Stat3 knockout mice were complicated with obesity and fatty liver, which may cause some secondary effects on liver regeneration. To study the direct role of STAT3 and to circumvent the problems of obesity and fatty liver in liver regeneration, we generated conditional STAT3 knockout in the liver (L-Stat3-/-) using a transthyretin-driven Cre-lox method. The L-Stat3-/- mice were born with the expected Mendelian frequency and showed no obesity or other obvious phenotype. After partial hepatectomy, mortality in the L-Stat3-/- mice was significantly higher than the littermate Stat3f/+ controls in the early time points (<24 h). Hepatocyte DNA synthesis in the survived L-Stat3-/- mice slightly decreased as compared with Stat3f/+ mice at 40 h after partial hepatectomy, whereas similar hepatocyte DNA synthesis was found at other time points and liver mass could be completely recovered in the L-Stat3-/- mice. In another model of liver regeneration induced by subcutaneous injection of carbon tetrachloride (CCl4), hepatocyte DNA synthesis in the CCl4-treated L-Stat3-/- mice also decreased as compared with Stat3f/+ mice at 40 h after injection but not at other time points. In addition, infiltration of neutrophils and monocyte increased in the liver of CCl4-treated L-Stat3-/- mice compared to wild-type mice. In conclusion, STAT3 is required for survival in the acute stage after 70% hepatectomy and plays a role in inflammatory reaction after hepatocyte necrosis. However, the hepatocytic STAT3 may have limited role in liver mass recovery although DNA synthesis may be impaired.

Original languageEnglish
Pages (from-to)1018-1028
Number of pages11
JournalLaboratory Investigation
Volume87
Issue number10
DOIs
StatePublished - Oct 2007

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STAT3 Transcription Factor
Liver Regeneration
Interleukin-6
Liver
DNA
Hepatocytes
Hepatectomy
Obesity
Fatty Liver
Prealbumin
Neutrophil Infiltration
Carbon Tetrachloride
Subcutaneous Injections
Knockout Mice
Monocytes
Necrosis
Phenotype
Injections
Mortality

Keywords

  • CCl
  • Conditional knockout
  • Liver regeneration
  • Partial hepatectomy
  • STAT

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Moh, A., Iwamoto, Y., Chai, G. X., Zhang, S. S. M., Kano, A., Yang, D. D., ... Fu, X. Y. (2007). Role of STAT3 in liver regeneration: Survival, DNA synthesis, inflammatory reaction and liver mass recovery. Laboratory Investigation, 87(10), 1018-1028. https://doi.org/10.1038/labinvest.3700630

Role of STAT3 in liver regeneration : Survival, DNA synthesis, inflammatory reaction and liver mass recovery. / Moh, Akira; Iwamoto, Yoshiki; Chai, Gui Xuan; Zhang, Samual Shao Min; Kano, Arihiro; Yang, Derek D.; Zhang, Wenjun; Wang, Jun; Jacoby, Joerg J.; Gao, Bin; Flavell, Richard A.; Fu, Xin Yuan.

In: Laboratory Investigation, Vol. 87, No. 10, 10.2007, p. 1018-1028.

Research output: Contribution to journalArticle

Moh, A, Iwamoto, Y, Chai, GX, Zhang, SSM, Kano, A, Yang, DD, Zhang, W, Wang, J, Jacoby, JJ, Gao, B, Flavell, RA & Fu, XY 2007, 'Role of STAT3 in liver regeneration: Survival, DNA synthesis, inflammatory reaction and liver mass recovery', Laboratory Investigation, vol. 87, no. 10, pp. 1018-1028. https://doi.org/10.1038/labinvest.3700630
Moh, Akira ; Iwamoto, Yoshiki ; Chai, Gui Xuan ; Zhang, Samual Shao Min ; Kano, Arihiro ; Yang, Derek D. ; Zhang, Wenjun ; Wang, Jun ; Jacoby, Joerg J. ; Gao, Bin ; Flavell, Richard A. ; Fu, Xin Yuan. / Role of STAT3 in liver regeneration : Survival, DNA synthesis, inflammatory reaction and liver mass recovery. In: Laboratory Investigation. 2007 ; Vol. 87, No. 10. pp. 1018-1028.
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