Role of surfactant protein A in the pathogenesis of tuberculosis in subjects with human immunodeficiency virus infection.

W. J. Martin, J. F. Downing, M. D. Williams, R. Pasula, Homer Twigg, J. R. Wright

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Abstract

Human immunodeficiency virus (HIV)-infected subjects are at increased risk for tuberculosis even before there is a significant loss of CD4 lymphocytes. A factor was found to be present in the bronchoalveolar lavage (BAL) of HIV-infected subjects that promoted the attachment of M. tuberculosis (MTB) organisms to alveolar macrophages (AMs). Using 51Cr-labeled MTB organisms, BAL from control subjects resulted in MTB attachment to AMs at 11.6% +/- 1.0%; in contrast, BAL from HIV-infected subjects increased attachment to 33.1% +/- 3.8% (P < 0.001). Surfactant protein A (SP-A) levels in BAL of normal controls was 1.9 +/- 0.3 micrograms/ml and was 5.5 +/- 0.4 micrograms/ml in the BAL of HIV-infected subjects (P < 0.01). When SP-A was removed by immunoprecipitation from the BAL of HIV-infected subjects, MTB attachment decreased from 33.1% +/- 3.8% to 11.3% +/- 0.4% (P < 0.001), a value identical to control levels. Exogenous human SP-A (5 micrograms/ml) was added back to the immunoprecipitated BAL and the enhanced attachment of MTB was restored. These data suggest that BAL from HIV-infected subjects contain a factor that facilitates MTB attachment to AMs, the first critical step in the establishment of infection. This factor appears to be SP-A.

Original languageEnglish
Pages (from-to)340-345
Number of pages6
JournalProceedings of the Association of American Physicians
Volume107
Issue number3
StatePublished - Oct 1995

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Pulmonary Surfactant-Associated Protein A
Bronchoalveolar Lavage
Virus Diseases
Tuberculosis
HIV
Alveolar Macrophages
Immunoprecipitation
Lymphocytes

ASJC Scopus subject areas

  • Medicine(all)

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Role of surfactant protein A in the pathogenesis of tuberculosis in subjects with human immunodeficiency virus infection. / Martin, W. J.; Downing, J. F.; Williams, M. D.; Pasula, R.; Twigg, Homer; Wright, J. R.

In: Proceedings of the Association of American Physicians, Vol. 107, No. 3, 10.1995, p. 340-345.

Research output: Contribution to journalArticle

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abstract = "Human immunodeficiency virus (HIV)-infected subjects are at increased risk for tuberculosis even before there is a significant loss of CD4 lymphocytes. A factor was found to be present in the bronchoalveolar lavage (BAL) of HIV-infected subjects that promoted the attachment of M. tuberculosis (MTB) organisms to alveolar macrophages (AMs). Using 51Cr-labeled MTB organisms, BAL from control subjects resulted in MTB attachment to AMs at 11.6{\%} +/- 1.0{\%}; in contrast, BAL from HIV-infected subjects increased attachment to 33.1{\%} +/- 3.8{\%} (P < 0.001). Surfactant protein A (SP-A) levels in BAL of normal controls was 1.9 +/- 0.3 micrograms/ml and was 5.5 +/- 0.4 micrograms/ml in the BAL of HIV-infected subjects (P < 0.01). When SP-A was removed by immunoprecipitation from the BAL of HIV-infected subjects, MTB attachment decreased from 33.1{\%} +/- 3.8{\%} to 11.3{\%} +/- 0.4{\%} (P < 0.001), a value identical to control levels. Exogenous human SP-A (5 micrograms/ml) was added back to the immunoprecipitated BAL and the enhanced attachment of MTB was restored. These data suggest that BAL from HIV-infected subjects contain a factor that facilitates MTB attachment to AMs, the first critical step in the establishment of infection. This factor appears to be SP-A.",
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