Role of the Ca2+ cycle in uncoupling of oxidative phosphorylation in liver mitochondria of cold-acclimated rats

Nicolay N. Brustovetsky, Evgeni I. Maevsky, Stella G. Kolaeva, Lubov S. Danilova, Nikita G. Solomonov

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

1. 1. Cold acclimation of Wistar rats for 2-4 weeks at about 3°C resulted in an increased respiration rate and a reduced ADP/O ratio in liver mitochondria. With increasing duration of acclimation up to 10-12 weeks, these parameters returned to a normal level. 2. 2. The increase in the respiration rate and the decline of the mitochondrial ADP/O ratio were associated with a significant activation of the electroneutral release of Ca2+. 3. 3. When the animals were acclimated for 10-12 weeks the rate of Ca2+ release reduced to control values. 4. 4. The addition of 1 μM ruthenium red resulted in a decrease in the rates of mitochondrial respiration in control and cold-acclimated rats to approximately equal values and in a partial restoration of the ADP/O ratio in liver mitochondria of rats kept in the cold for 2-4 weeks. 5. 5. The respiratory activity of mitochondria isolated in the presence of 1 mM EGTA was unaffected by ruthenium red.

Original languageEnglish (US)
Pages (from-to)545-547
Number of pages3
JournalComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume82
Issue number3
DOIs
StatePublished - 1985

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Mitochondria
Liver Mitochondrion
Oxidative Phosphorylation
Respiratory Rate
Liver
Adenosine Diphosphate
Rats
Ruthenium Red
Acclimatization
Egtazic Acid
Restoration
Wistar Rats
Animals
Chemical activation

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology

Cite this

Role of the Ca2+ cycle in uncoupling of oxidative phosphorylation in liver mitochondria of cold-acclimated rats. / Brustovetsky, Nicolay N.; Maevsky, Evgeni I.; Kolaeva, Stella G.; Danilova, Lubov S.; Solomonov, Nikita G.

In: Comparative Biochemistry and Physiology -- Part B: Biochemistry and, Vol. 82, No. 3, 1985, p. 545-547.

Research output: Contribution to journalArticle

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AU - Danilova, Lubov S.

AU - Solomonov, Nikita G.

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N2 - 1. 1. Cold acclimation of Wistar rats for 2-4 weeks at about 3°C resulted in an increased respiration rate and a reduced ADP/O ratio in liver mitochondria. With increasing duration of acclimation up to 10-12 weeks, these parameters returned to a normal level. 2. 2. The increase in the respiration rate and the decline of the mitochondrial ADP/O ratio were associated with a significant activation of the electroneutral release of Ca2+. 3. 3. When the animals were acclimated for 10-12 weeks the rate of Ca2+ release reduced to control values. 4. 4. The addition of 1 μM ruthenium red resulted in a decrease in the rates of mitochondrial respiration in control and cold-acclimated rats to approximately equal values and in a partial restoration of the ADP/O ratio in liver mitochondria of rats kept in the cold for 2-4 weeks. 5. 5. The respiratory activity of mitochondria isolated in the presence of 1 mM EGTA was unaffected by ruthenium red.

AB - 1. 1. Cold acclimation of Wistar rats for 2-4 weeks at about 3°C resulted in an increased respiration rate and a reduced ADP/O ratio in liver mitochondria. With increasing duration of acclimation up to 10-12 weeks, these parameters returned to a normal level. 2. 2. The increase in the respiration rate and the decline of the mitochondrial ADP/O ratio were associated with a significant activation of the electroneutral release of Ca2+. 3. 3. When the animals were acclimated for 10-12 weeks the rate of Ca2+ release reduced to control values. 4. 4. The addition of 1 μM ruthenium red resulted in a decrease in the rates of mitochondrial respiration in control and cold-acclimated rats to approximately equal values and in a partial restoration of the ADP/O ratio in liver mitochondria of rats kept in the cold for 2-4 weeks. 5. 5. The respiratory activity of mitochondria isolated in the presence of 1 mM EGTA was unaffected by ruthenium red.

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