Role of the pituitary and neonatal androgenic imprinting in the hormonal regulation of liver alcohol dehydrogenase activity

David Crabb, William F. Bosron, Li Ting-Kai Li

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Liver alcohol dehydrogenase activity is increased by thyroidectomy, orchidectomy, or hypophysectomy. We investigated the mechanisms of these hormonal effects by examining the effects of testosterone, dexamethasone and thyroid hormone on liver alcohol dehydrogenase activity in hypophysectomized rats and in cultured hepatocytes, and the effect of administration of androgens to neonatal female rats. Testosterone did not lower alcohol dehydrogenase activity in hypophysectomized rats, whereas dexamethasone and thyroxine produced moderate decreases in activity. Triiodothyronine reduced alcohol dehydrogenase activity of cultured hepatocytes from male and hypothyroid female rats in a dose-dependent fashion, confirming that thyroid hormone had pituitary-independent effects on the enzyme activity. Dexamethasone was required for the expression of alcohol dehydrogenase activity in cultured cells, and it increased the enzyme activity when present at supraphysiologic concentrations. Treatment of neonatal female rats with testosterone reduced the activity of the enzyme in adulthood. The difference in alcohol dehydrogenase activity in adult male and female rats appears to be determined in part by neonatal imprinting by androgens and in part by an effect of testosterone that is either mediated by or dependent upon the pituitary. Thyroid hormone reduces alcohol dehydrogenase activity by a direct effect on the liver.

Original languageEnglish
Pages (from-to)1527-1532
Number of pages6
JournalBiochemical Pharmacology
Volume35
Issue number9
DOIs
StatePublished - May 1 1986

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Alcohol Dehydrogenase
Liver
Rats
Testosterone
Thyroid Hormones
Dexamethasone
Enzyme activity
Androgens
Hepatocytes
Enzymes
Hypophysectomy
Orchiectomy
Thyroidectomy
Triiodothyronine
Thyroxine
Cultured Cells
Cells

ASJC Scopus subject areas

  • Pharmacology

Cite this

Role of the pituitary and neonatal androgenic imprinting in the hormonal regulation of liver alcohol dehydrogenase activity. / Crabb, David; Bosron, William F.; Ting-Kai Li, Li.

In: Biochemical Pharmacology, Vol. 35, No. 9, 01.05.1986, p. 1527-1532.

Research output: Contribution to journalArticle

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