Role of the POZ Zinc Finger Transcription Factor FBI-1 in Human and Murine Adipogenesis

Matthias Laudes, Constantinos Christodoulides, Ciaran Sewter, Justin J. Rochford, Robert Considine, Jaswinder K. Sethi, Antonio Vidal-Puig, Stephen O'Rahilly

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Poxvirus zinc finger (POZ) zinc finger domain transcription factors have been shown to play a role in the control of growth arrest and differentiation in several types of mesenchymal cells but not, as yet, adipocytes. We found that a POZ domain protein, factor that binds to inducer of short transcripts-1 (FBI-1), was induced during both murine and human preadipocyte differentiation with maximal expression levels seen at days 2-4. FBI-1 mRNA was expressed in human adipose tissue with the highest levels found in samples from morbidly obese subjects. Murine cell lines constitutively expressing FBI-1 showed evidence for accelerated adipogenesis with earlier induction of markers of differentiation and enhanced lipid accumulation, suggesting that FBI-1 may be an active participant in the differentiation process. Consistent with the properties of this family of proteins in other cell systems, 3T3L1 cells stably overexpressing FBI-1 showed reduced DNA synthesis and reduced expression of cyclin A, cyclin-dependent kinase 2, and p107, proteins known to be involved in the regulation of mitotic clonal expansion. In addition, FBI-1 reduced the transcriptional activity of the cyclin A promoter. Thus, FBI-1, a POZ zinc finger transcription factor, is induced during the early phases of human and murine preadipocyte differentiation where it may contribute to adipogenesis through influencing the switch from cellular proliferation to terminal differentiation.

Original languageEnglish
Pages (from-to)11711-11718
Number of pages8
JournalJournal of Biological Chemistry
Volume279
Issue number12
DOIs
StatePublished - Mar 19 2004

Fingerprint

Poxviridae
Adipogenesis
Zinc Fingers
Zinc
Transcription Factors
Cyclin A
Cyclin-Dependent Kinase 2
Proteins
Differentiation Antigens
Adipocytes
Adipose Tissue
Cell Proliferation
Lipids
Cell Line
Messenger RNA
Cells
Switches
DNA
Tissue
Growth

ASJC Scopus subject areas

  • Biochemistry

Cite this

Laudes, M., Christodoulides, C., Sewter, C., Rochford, J. J., Considine, R., Sethi, J. K., ... O'Rahilly, S. (2004). Role of the POZ Zinc Finger Transcription Factor FBI-1 in Human and Murine Adipogenesis. Journal of Biological Chemistry, 279(12), 11711-11718. https://doi.org/10.1074/jbc.M310240200

Role of the POZ Zinc Finger Transcription Factor FBI-1 in Human and Murine Adipogenesis. / Laudes, Matthias; Christodoulides, Constantinos; Sewter, Ciaran; Rochford, Justin J.; Considine, Robert; Sethi, Jaswinder K.; Vidal-Puig, Antonio; O'Rahilly, Stephen.

In: Journal of Biological Chemistry, Vol. 279, No. 12, 19.03.2004, p. 11711-11718.

Research output: Contribution to journalArticle

Laudes, M, Christodoulides, C, Sewter, C, Rochford, JJ, Considine, R, Sethi, JK, Vidal-Puig, A & O'Rahilly, S 2004, 'Role of the POZ Zinc Finger Transcription Factor FBI-1 in Human and Murine Adipogenesis', Journal of Biological Chemistry, vol. 279, no. 12, pp. 11711-11718. https://doi.org/10.1074/jbc.M310240200
Laudes, Matthias ; Christodoulides, Constantinos ; Sewter, Ciaran ; Rochford, Justin J. ; Considine, Robert ; Sethi, Jaswinder K. ; Vidal-Puig, Antonio ; O'Rahilly, Stephen. / Role of the POZ Zinc Finger Transcription Factor FBI-1 in Human and Murine Adipogenesis. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 12. pp. 11711-11718.
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