RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation

Zuyao Ni, Chao Xu, Xinghua Guo, Gerald O. Hunter, Olga V. Kuznetsova, Wolfram Tempel, Edyta Marcon, Guoqing Zhong, Hongbo Guo, Wei Hung William Kuo, Joyce Li, Peter Young, Jonathan B. Olsen, Cuihong Wan, Peter Loppnau, Majida El Bakkouri, Guillermo A. Senisterra, Hao He, Haiming Huang, Sachdev S. Sidhu & 6 others Andrew Emili, Shona Murphy, Amber Mosley, Cheryl H. Arrowsmith, Jinrong Min, Jack F. Greenblatt

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The RNA polymerase II (RNAPII) C-terminal domain (CTD) heptapeptide repeats (1-YSPTSPS-7) undergo dynamic phosphorylation and dephosphorylation during the transcription cycle to recruit factors that regulate transcription, RNA processing and chromatin modification. We show here that RPRD1A and RPRD1B form homodimers and heterodimers through their coiled-coil domains and interact preferentially via CTD-interaction domains (CIDs) with RNAPII CTD repeats phosphorylated at S2 and S7. Crystal structures of the RPRD1A, RPRD1B and RPRD2 CIDs, alone and in complex with RNAPII CTD phosphoisoforms, elucidate the molecular basis of CTD recognition. In an example of cross-talk between different CTD modifications, our data also indicate that RPRD1A and RPRD1B associate directly with RPAP2 phosphatase and, by interacting with CTD repeats where phospho-S2 and/or phospho-S7 bracket a phospho-S5 residue, serve as CTD scaffolds to coordinate the dephosphorylation of phospho-S5 by RPAP2.

Original languageEnglish
Pages (from-to)686-695
Number of pages10
JournalNature Structural and Molecular Biology
Volume21
Issue number8
DOIs
StatePublished - 2014

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RNA Polymerase III
RNA Polymerase II
ethyl-2-methylthio-4-methyl-5-pyrimidine carboxylate
Terminal Repeat Sequences
Phosphoric Monoester Hydrolases
Chromatin
Transcription Factors
Phosphorylation
RNA

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this

Ni, Z., Xu, C., Guo, X., Hunter, G. O., Kuznetsova, O. V., Tempel, W., ... Greenblatt, J. F. (2014). RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. Nature Structural and Molecular Biology, 21(8), 686-695. https://doi.org/10.1038/nsmb.2853

RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. / Ni, Zuyao; Xu, Chao; Guo, Xinghua; Hunter, Gerald O.; Kuznetsova, Olga V.; Tempel, Wolfram; Marcon, Edyta; Zhong, Guoqing; Guo, Hongbo; Kuo, Wei Hung William; Li, Joyce; Young, Peter; Olsen, Jonathan B.; Wan, Cuihong; Loppnau, Peter; El Bakkouri, Majida; Senisterra, Guillermo A.; He, Hao; Huang, Haiming; Sidhu, Sachdev S.; Emili, Andrew; Murphy, Shona; Mosley, Amber; Arrowsmith, Cheryl H.; Min, Jinrong; Greenblatt, Jack F.

In: Nature Structural and Molecular Biology, Vol. 21, No. 8, 2014, p. 686-695.

Research output: Contribution to journalArticle

Ni, Z, Xu, C, Guo, X, Hunter, GO, Kuznetsova, OV, Tempel, W, Marcon, E, Zhong, G, Guo, H, Kuo, WHW, Li, J, Young, P, Olsen, JB, Wan, C, Loppnau, P, El Bakkouri, M, Senisterra, GA, He, H, Huang, H, Sidhu, SS, Emili, A, Murphy, S, Mosley, A, Arrowsmith, CH, Min, J & Greenblatt, JF 2014, 'RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation', Nature Structural and Molecular Biology, vol. 21, no. 8, pp. 686-695. https://doi.org/10.1038/nsmb.2853
Ni, Zuyao ; Xu, Chao ; Guo, Xinghua ; Hunter, Gerald O. ; Kuznetsova, Olga V. ; Tempel, Wolfram ; Marcon, Edyta ; Zhong, Guoqing ; Guo, Hongbo ; Kuo, Wei Hung William ; Li, Joyce ; Young, Peter ; Olsen, Jonathan B. ; Wan, Cuihong ; Loppnau, Peter ; El Bakkouri, Majida ; Senisterra, Guillermo A. ; He, Hao ; Huang, Haiming ; Sidhu, Sachdev S. ; Emili, Andrew ; Murphy, Shona ; Mosley, Amber ; Arrowsmith, Cheryl H. ; Min, Jinrong ; Greenblatt, Jack F. / RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. In: Nature Structural and Molecular Biology. 2014 ; Vol. 21, No. 8. pp. 686-695.
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AU - Ni, Zuyao

AU - Xu, Chao

AU - Guo, Xinghua

AU - Hunter, Gerald O.

AU - Kuznetsova, Olga V.

AU - Tempel, Wolfram

AU - Marcon, Edyta

AU - Zhong, Guoqing

AU - Guo, Hongbo

AU - Kuo, Wei Hung William

AU - Li, Joyce

AU - Young, Peter

AU - Olsen, Jonathan B.

AU - Wan, Cuihong

AU - Loppnau, Peter

AU - El Bakkouri, Majida

AU - Senisterra, Guillermo A.

AU - He, Hao

AU - Huang, Haiming

AU - Sidhu, Sachdev S.

AU - Emili, Andrew

AU - Murphy, Shona

AU - Mosley, Amber

AU - Arrowsmith, Cheryl H.

AU - Min, Jinrong

AU - Greenblatt, Jack F.

PY - 2014

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N2 - The RNA polymerase II (RNAPII) C-terminal domain (CTD) heptapeptide repeats (1-YSPTSPS-7) undergo dynamic phosphorylation and dephosphorylation during the transcription cycle to recruit factors that regulate transcription, RNA processing and chromatin modification. We show here that RPRD1A and RPRD1B form homodimers and heterodimers through their coiled-coil domains and interact preferentially via CTD-interaction domains (CIDs) with RNAPII CTD repeats phosphorylated at S2 and S7. Crystal structures of the RPRD1A, RPRD1B and RPRD2 CIDs, alone and in complex with RNAPII CTD phosphoisoforms, elucidate the molecular basis of CTD recognition. In an example of cross-talk between different CTD modifications, our data also indicate that RPRD1A and RPRD1B associate directly with RPAP2 phosphatase and, by interacting with CTD repeats where phospho-S2 and/or phospho-S7 bracket a phospho-S5 residue, serve as CTD scaffolds to coordinate the dephosphorylation of phospho-S5 by RPAP2.

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