Rrp2, a prokaryotic enhancer-like binding protein, is essential for viability of Borrelia burgdorferi

Ashley M. Groshong, Nora E. Gibbons, X. Yang, Jon S. Blevins

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The Lyme disease spirochete, Borrelia burgdorferi, exists in two diverse niches (i.e., an arthropod tick vector and mammalian host) during its enzootic life cycle. To effectively adapt to these unique environments, the bacterium alters the expression of numerous genes, including several major outer surface (lipo)proteins that are required for infection and transmission. An enhancerbinding protein (EBP), known as Rrp2, is one identified activator of the RpoN/RpoS alternative sigma factor cascade. Because initial efforts to generate an rrp2 deletion strain were unsuccessful, the role of Rrp2 in the activation of the RpoN/RpoS pathway was first defined using a strain of B. burgdorferi carrying an rrp2 point mutant that was defective in its ability to activate RpoNdependent transcription. The fact that subsequent attempts to disrupt rrp2 have also been unsuccessful has led investigators to hypothesize that Rrp2 has other undefined functions which are essential for B. burgdorferi survival and independent of its EBP function. We used a lac-based inducible expression system to generate a conditional rrp2 mutant in virulent B. burgdorferi. In this strain, an isopropyl-β-D-thiogalactopyranoside-inducible copy of the rrp2 gene is expressed in trans from a borrelial shuttle vector. We found that the chromosomal copy of rrp2 could be inactivated only when rrp2 was induced, and the maintenance of rrp2 expression was required for the growth of the mutants. In addition, the overexpression of rrp2 is detrimental to B. burgdorferi growth in a manner that is independent of the RpoN/RpoS pathway. These studies provide the first direct evidence that rrp2 is an essential gene in B. burgdorferi.

Original languageEnglish
Pages (from-to)3336-3342
Number of pages7
JournalJournal of Bacteriology
Volume194
Issue number13
DOIs
StatePublished - Jul 2012

Fingerprint

Borrelia burgdorferi
Carrier Proteins
Arthropod Vectors
Thiogalactosides
Sigma Factor
Genetic Vectors
Infectious Disease Transmission
Essential Genes
Ticks
Growth
Life Cycle Stages
Membrane Proteins
Proteins
Maintenance
Research Personnel
Bacteria
Gene Expression
Genes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Rrp2, a prokaryotic enhancer-like binding protein, is essential for viability of Borrelia burgdorferi. / Groshong, Ashley M.; Gibbons, Nora E.; Yang, X.; Blevins, Jon S.

In: Journal of Bacteriology, Vol. 194, No. 13, 07.2012, p. 3336-3342.

Research output: Contribution to journalArticle

Groshong, Ashley M. ; Gibbons, Nora E. ; Yang, X. ; Blevins, Jon S. / Rrp2, a prokaryotic enhancer-like binding protein, is essential for viability of Borrelia burgdorferi. In: Journal of Bacteriology. 2012 ; Vol. 194, No. 13. pp. 3336-3342.
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