Rtr1 Is a CTD Phosphatase that Regulates RNA Polymerase II during the Transition from Serine 5 to Serine 2 Phosphorylation

Amber Mosley, Samantha G. Pattenden, Michael Carey, Swaminathan Venkatesh, Joshua M. Gilmore, Laurence Florens, Jerry L. Workman, Michael P. Washburn

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

Messenger RNA processing is coupled to RNA polymerase II (RNAPII) transcription through coordinated recruitment of accessory proteins to the Rpb1 C-terminal domain (CTD). Dynamic changes in CTD phosphorylation during transcription elongation are responsible for their recruitment, with serine 5 phosphorylation (S5-P) occurring toward the 5′ end of genes and serine 2 phosphorylation (S2-P) occurring toward the 3′ end. The proteins responsible for regulation of the transition state between S5-P and S2-P CTD remain elusive. We show that a conserved protein of unknown function, Rtr1, localizes within coding regions, with maximum levels of enrichment occurring between the peaks of S5-P and S2-P RNAPII. Upon deletion of Rtr1, the S5-P form of RNAPII accumulates in both whole-cell extracts and throughout coding regions; additionally, RNAPII transcription is decreased, and termination defects are observed. Functional characterization of Rtr1 reveals its role as a CTD phosphatase essential for the S5-to-S2-P transition.

Original languageEnglish (US)
Pages (from-to)168-178
Number of pages11
JournalMolecular Cell
Volume34
Issue number2
DOIs
StatePublished - Apr 24 2009
Externally publishedYes

Fingerprint

RNA Polymerase II
Phosphoric Monoester Hydrolases
Serine
Phosphorylation
Proteins
Cell Extracts
Messenger RNA

Keywords

  • DNA
  • PROTEINS

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Rtr1 Is a CTD Phosphatase that Regulates RNA Polymerase II during the Transition from Serine 5 to Serine 2 Phosphorylation. / Mosley, Amber; Pattenden, Samantha G.; Carey, Michael; Venkatesh, Swaminathan; Gilmore, Joshua M.; Florens, Laurence; Workman, Jerry L.; Washburn, Michael P.

In: Molecular Cell, Vol. 34, No. 2, 24.04.2009, p. 168-178.

Research output: Contribution to journalArticle

Mosley, A, Pattenden, SG, Carey, M, Venkatesh, S, Gilmore, JM, Florens, L, Workman, JL & Washburn, MP 2009, 'Rtr1 Is a CTD Phosphatase that Regulates RNA Polymerase II during the Transition from Serine 5 to Serine 2 Phosphorylation', Molecular Cell, vol. 34, no. 2, pp. 168-178. https://doi.org/10.1016/j.molcel.2009.02.025
Mosley, Amber ; Pattenden, Samantha G. ; Carey, Michael ; Venkatesh, Swaminathan ; Gilmore, Joshua M. ; Florens, Laurence ; Workman, Jerry L. ; Washburn, Michael P. / Rtr1 Is a CTD Phosphatase that Regulates RNA Polymerase II during the Transition from Serine 5 to Serine 2 Phosphorylation. In: Molecular Cell. 2009 ; Vol. 34, No. 2. pp. 168-178.
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