SAA suppression of immune response in vitro: Evidence for an effect on T cell-macrophage interaction

M. A. Aldo-Benson, Merrill Benson

Research output: Contribution to journalArticle

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Abstract

SAA has been shown previously to inhibit the in vitro antibody response of normal murine spleen cells to SRBC. This effect is nonspecific but does not directly suppress B cells. To evaluate the mechanisms of this suppression, the in vitro antibody response to SRBC was studied using cells from cytoxan-treated mice. No evidence for preformed suppressor cells was found. Also, preincubation of normal cells with SAA failed to reveal evidence for the generation of suppressor cells in vitro. The addition of 2-mercaptoethanol to cultures completely reversed the suppression caused by SAA. Addition of either normal T cells or normal macrophages to cultures partially reversed suppression caused by SAA, but only a combination of T cells and macrophages could completely reverse the suppression. These data are consistent with an effect of SAA on macrophage- T cell interaction in the generation of the immune response to T-dependent antigen. No evidence for the participation of suppressor cells as has been demonstrated for α-fetoprotein was found.

Original languageEnglish
Pages (from-to)2390-2392
Number of pages3
JournalJournal of Immunology
Volume128
Issue number6
StatePublished - 1982

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Cell Communication
Macrophages
T-Lymphocytes
Antibody Formation
Fetal Proteins
Mercaptoethanol
Viral Tumor Antigens
Cyclophosphamide
In Vitro Techniques
B-Lymphocytes
Spleen

ASJC Scopus subject areas

  • Immunology

Cite this

SAA suppression of immune response in vitro : Evidence for an effect on T cell-macrophage interaction. / Aldo-Benson, M. A.; Benson, Merrill.

In: Journal of Immunology, Vol. 128, No. 6, 1982, p. 2390-2392.

Research output: Contribution to journalArticle

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