Salivary epithelial cells as model to study immune response against cutaneous pathogens

Thais C. Negrini, Rodrigo A. Arthur, Robert A. Waeiss, Iracilda Z. Carlosa, Mythily Srinivasan

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The human skin not only provides passive protection as a physical barrier against external injury, but also mediates active surveillance via epidermal cell surface receptors that recognize and respond to potential invaders. Primary keratinocytes and immortalized cell lines, the commonly used sources to investigate immune responses of cutaneous epithelium are often difficult to obtain and/or potentially exhibit changes in cellular genetic make-up. Here we investigated the possibility of using salivary epithelial cells (SEC) to evaluate the host response to cutaneous microbes. Elevated secretion of IFN-γ and IL-12 was observed in the SEC stimulated with Staphylococcus aureus, a transient pathogen of the skin, as mono species biofilm as compared to SEC stimulated with a commensal microbe, the Staphylococcus epidermidis. Co-culture of the SEC with both microbes as dual species biofilm elicited maximum cytokine response. Stimulation with S. aureus alone but not with S. epidermidis alone induced maximum toll-like receptor-2 (TLR-2) expression in the SEC. Exposure to dual species biofilm induced a sustained upregulation of TLR-2 in the SEC for up to an hour. The data support novel application of the SEC as efficient biospecimen that may be used to investigate personalized response to cutaneous microflora.

Original languageEnglish (US)
Pages (from-to)48-51
Number of pages4
JournalClinical and Translational Science
Volume7
Issue number1
DOIs
StatePublished - Feb 2014

Fingerprint

Pathogens
Epithelial Cells
Skin
Biofilms
Toll-Like Receptor 2
Staphylococcus epidermidis
Staphylococcus aureus
Architectural Accessibility
Cell Surface Receptors
Interleukin-12
Coculture Techniques
Keratinocytes
Up-Regulation
Epithelium
Cells
Cytokines
Cell Line
Wounds and Injuries

Keywords

  • Biofilm
  • Cytokines
  • Salivary epithelial cells
  • TLR-2

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Neuroscience(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Salivary epithelial cells as model to study immune response against cutaneous pathogens. / Negrini, Thais C.; Arthur, Rodrigo A.; Waeiss, Robert A.; Carlosa, Iracilda Z.; Srinivasan, Mythily.

In: Clinical and Translational Science, Vol. 7, No. 1, 02.2014, p. 48-51.

Research output: Contribution to journalArticle

Negrini, Thais C. ; Arthur, Rodrigo A. ; Waeiss, Robert A. ; Carlosa, Iracilda Z. ; Srinivasan, Mythily. / Salivary epithelial cells as model to study immune response against cutaneous pathogens. In: Clinical and Translational Science. 2014 ; Vol. 7, No. 1. pp. 48-51.
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