Salivary IgA responses to Porphyromonas gingivalis in the cynomolgus monkey. I. Total IgA and IgA antibody levels to P. gingivalis

Steven Blanchard, Sarah E. Cox, Jeffrey L. Ebersole

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Porphyromonas gingivalis has been associated with the subgingival plaque of advancing disease lesions in various types of periodontitis. Additionally, this species of oral microorganism has been found to increase dramatically in ligature‐induced periodontitis in nonhuman primates (Macaca fascicularis) and has recently been shown to induce progressing disease when implanted into the subgingival plaque in this animal model. Although systemic antibody responses have been demonstrated to P. gingivalis in both human and nonhuman primate with periodontitis, no information is available on the oral secretory IgA antibody response to this bacteria. This report describes the methods for reproducible collection of salivary secretions from cynomolgus monkeys and the development of methods for analyzing salivary IgA levels and specific IgA antibody in the saliva reactive with P. gingivalis. Purification of monkey salivary IgA allowed quantification of IgA using an enzyme‐linked immunosorbent assay (ELISA). Estimation of total IgA levels in saliva showed approximately a 20% greater level of IgA in whole versus parotid saliva from a group of 13 monkeys, with a 2–3 fold variation in levels among this group of animals. Naturally occurring salivary IgA antibody to P. gingivalis, as measured by ELISA, were routinely detectable but low in whole saliva; however, many of the parotid saliva specimens collected exhibited negligible levels of antibody to this microorganism. The IgA antibody in whole saliva showed nearly an 18‐fold variation among the samples from the monkeys. Correlational analyses indicated that, although there was a positive relationship between antibody levels in whole and parotid saliva, the majority of natural IgA antibody in whole saliva appears to be derived from other sources. Additionally, a positive correlation between IgA antibody to P. gingivalis and total IgA levels in parotid saliva indicated a lack of a selective secretory immune response to P. gingivalis in the major salivary glands of normal monkeys. The results of these baseline studies of the nonhuman primate secretory immune responses show that naturally occurring salivary IgA antibody to P. gingivalis is low and the methods developed should facilitate future immunization experiments that can examine the interaction of salivary IgA antibody with the emergence and transmission of P. gingivalis within the oral cavity.

Original languageEnglish (US)
Pages (from-to)341-349
Number of pages9
JournalOral Microbiology and Immunology
Volume6
Issue number6
DOIs
StatePublished - Jan 1 1991
Externally publishedYes

Fingerprint

Porphyromonas gingivalis
Macaca fascicularis
Immunoglobulin A
Saliva
Antibodies
Haplorhini
Periodontitis
Primates
Immunosorbents
Antibody Formation
Secretory Immunoglobulin A
Salivary Glands
Mouth
Immunization

Keywords

  • ELISA
  • IgA
  • monkey
  • periodontal disease
  • Porphyromonas gingivalis
  • salivary antibody

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Dentistry(all)
  • Microbiology (medical)

Cite this

Salivary IgA responses to Porphyromonas gingivalis in the cynomolgus monkey. I. Total IgA and IgA antibody levels to P. gingivalis. / Blanchard, Steven; Cox, Sarah E.; Ebersole, Jeffrey L.

In: Oral Microbiology and Immunology, Vol. 6, No. 6, 01.01.1991, p. 341-349.

Research output: Contribution to journalArticle

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