SaOS2 osteosarcoma cells as an in vitro model for studying the transition of human osteoblasts to osteocytes

Matthew Prideaux, Asiri R. Wijenayaka, Duminda D. Kumarasinghe, Renee T. Ormsby, Andreas Evdokiou, David M. Findlay, Gerald J. Atkins

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The central importance of osteocytes in regulating bone homeostasis is becoming increasingly apparent. However, the study of these cells has been restricted by the relative paucity of cell line models, especially those of human origin. Therefore, we investigated the extent to which SaOS2 human osteosarcoma cells can differentiate into osteocyte-like cells. During culture under the appropriate mineralising conditions, SaOS2 cells reproducibly synthesised a bone-like mineralised matrix and temporally expressed the mature osteocyte marker genes SOST, DMP1, PHEX and MEPE and down-regulated expression of RUNX2 and COL1A1. SaOS2 cells cultured in 3D collagen gels acquired a dendritic morphology, characteristic of osteocytes, with multiple interconnecting cell processes. These findings suggest that SaOS2 cells have the capacity to differentiate into mature osteocyte-like cells under mineralising conditions. PTH treatment of SaOS2 cells resulted in strong down-regulation of SOST mRNA expression at all time points tested. Interestingly, PTH treatment resulted in the up-regulation of RANKL mRNA expression only at earlier stages of differentiation. These findings suggest that the response to PTH is dependent on the differentiation stage of the osteoblast/osteocyte. Together, our results demonstrate that SaOS2 cells can be used as a human model to investigate responses to osteotropic stimuli throughout differentiation to a mature osteocyte-like stage.

Original languageEnglish (US)
Pages (from-to)183-193
Number of pages11
JournalCalcified Tissue International
Volume95
Issue number2
DOIs
StatePublished - 2014
Externally publishedYes

Fingerprint

Osteocytes
Osteosarcoma
Osteoblasts
In Vitro Techniques
Bone and Bones
Messenger RNA
Cultured Cells
Homeostasis
Up-Regulation
Collagen
Down-Regulation
Gels
Cell Line

Keywords

  • Cell culture
  • Differentiation
  • Osteoblast
  • Osteocyte
  • PTH
  • RANKL
  • SaOS2
  • SOST

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Medicine(all)

Cite this

Prideaux, M., Wijenayaka, A. R., Kumarasinghe, D. D., Ormsby, R. T., Evdokiou, A., Findlay, D. M., & Atkins, G. J. (2014). SaOS2 osteosarcoma cells as an in vitro model for studying the transition of human osteoblasts to osteocytes. Calcified Tissue International, 95(2), 183-193. https://doi.org/10.1007/s00223-014-9879-y

SaOS2 osteosarcoma cells as an in vitro model for studying the transition of human osteoblasts to osteocytes. / Prideaux, Matthew; Wijenayaka, Asiri R.; Kumarasinghe, Duminda D.; Ormsby, Renee T.; Evdokiou, Andreas; Findlay, David M.; Atkins, Gerald J.

In: Calcified Tissue International, Vol. 95, No. 2, 2014, p. 183-193.

Research output: Contribution to journalArticle

Prideaux, M, Wijenayaka, AR, Kumarasinghe, DD, Ormsby, RT, Evdokiou, A, Findlay, DM & Atkins, GJ 2014, 'SaOS2 osteosarcoma cells as an in vitro model for studying the transition of human osteoblasts to osteocytes', Calcified Tissue International, vol. 95, no. 2, pp. 183-193. https://doi.org/10.1007/s00223-014-9879-y
Prideaux, Matthew ; Wijenayaka, Asiri R. ; Kumarasinghe, Duminda D. ; Ormsby, Renee T. ; Evdokiou, Andreas ; Findlay, David M. ; Atkins, Gerald J. / SaOS2 osteosarcoma cells as an in vitro model for studying the transition of human osteoblasts to osteocytes. In: Calcified Tissue International. 2014 ; Vol. 95, No. 2. pp. 183-193.
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