Sarcoplasmic reticulum Ca2+ release in neonatal rat cardiac myocytes

Ulrich Gergs, Uwe Kirchhefer, Jan Buskase, Katharina Kiele-Dunsche, Igor B. Buchwalow, Larry R. Jones, Wilhelm Schmitz, Otto Traub, Joachim Neumann

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

In the neonatal mammalian heart, the role of ryanodine receptor (=Ca2+ release channel)-mediated sarcoplasmic reticulum (SR) Ca2+ release for excitation-contraction coupling is still a matter of debate. Using an adenoviral system, we overexpressed separately the junctional SR proteins triadin, junctin, and calsequestrin, which are probably involved in regulation of ryanodine receptor function. Infection of neonatal rat cardiac myocytes with triadin, junctin, or calsequestrin viruses, controlled by green fluorescent protein expression, resulted in an increased protein level of the corresponding transgenes. Measurement of Ca2+ transients of infected cardiac myocytes revealed unchanged peak amplitudes under basal conditions but with overexpression of calsequestrin and triadin caffeine-releasable SR Ca2+ content was increased. Our results demonstrate that an increased expression of triadin or calsequestrin is associated with an increased SR Ca2+ storage but unchanged Ca2+ signaling in neonatal rat cardiac myocytes. This is consistent with an ancillary role of the sarcoplasmic reticulum in excitation-contraction coupling in the developing mammalian heart.

Original languageEnglish (US)
Pages (from-to)682-688
Number of pages7
JournalJournal of Molecular and Cellular Cardiology
Volume51
Issue number5
DOIs
StatePublished - Nov 1 2011

Keywords

  • Adenovirus
  • Calcium handling
  • Calsequestrin
  • Junctin
  • Triadin

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

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    Gergs, U., Kirchhefer, U., Buskase, J., Kiele-Dunsche, K., Buchwalow, I. B., Jones, L. R., Schmitz, W., Traub, O., & Neumann, J. (2011). Sarcoplasmic reticulum Ca2+ release in neonatal rat cardiac myocytes. Journal of Molecular and Cellular Cardiology, 51(5), 682-688. https://doi.org/10.1016/j.yjmcc.2011.08.007