Scanning electron microscopy of kidney cells in culture

surface features of polarized epithelia.

J. A. McAteer, G. S. Dougherty, K. D. Gardner, Andrew Evan

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We have used scanning electron microscopy (SEM) to examine the surface morphology of the renal epithelial cell lines MDCK and LLC-PKl to determine the influence of alternative culture substrate conditions on cell polarity. We observed that regardless of physical culture conditions, cells established and maintained polarity, expressed by the characteristics of apical and basal surfaces. Culture conditions did, however, influence the orientation of cell polarity in vitro. MDCK cells were grown within collagen gel, in which individual cells exhibited clonal growth to form fluid-filled epithelial cysts. The cells of MDCK-cysts were polarized with apical surface facing the lumen and basal surface against the surrounding collagen gel. This configuration made it possible to gain direct visual access, by SEM, to the basal surface by removing the supportive collagen lattice. The apical surface of MDCK-cysts was lined by short microvilli. Each cell possessed a solitary cilium. In comparison, the basal surface had few appendages, although cell boundaries were marked by interdigitating short processes. LLC-PKl cells in monolayer culture bore solitary cilia and long microvilli at their apical surface. The basal surface of cells involved in dome formation was observed to possess only a sparse population of short, blunt processes. When LLC-PKl cells were raised in stationary suspension culture or in monolayer atop non-culture grade plastic, they formed cysts with the cell apex facing the surrounding medium. These cells showed variable apical morphology. The cells of large, highly expanded cysts were often attenuated and had a relatively smooth apical surface. The basal surface of cells of fractured LLC-PKl cysts commonly was also smooth, without prominent appendages.

Original languageEnglish
Pages (from-to)1135-1150
Number of pages16
JournalScanning Electron Microscopy
Issue numberPt 3
StatePublished - 1986
Externally publishedYes

Fingerprint

Electron Scanning Microscopy
Epithelium
Cell Culture Techniques
Kidney
Scanning electron microscopy
Cysts
Collagen
Cell Polarity
Madin Darby Canine Kidney Cells
Cilia
Microvilli
Monolayers
Gels
Domes
Plastics
Surface morphology
Suspensions
Epithelial Cells
Cell Line
Fluids

ASJC Scopus subject areas

  • Biophysics
  • Control and Systems Engineering

Cite this

Scanning electron microscopy of kidney cells in culture : surface features of polarized epithelia. / McAteer, J. A.; Dougherty, G. S.; Gardner, K. D.; Evan, Andrew.

In: Scanning Electron Microscopy, No. Pt 3, 1986, p. 1135-1150.

Research output: Contribution to journalArticle

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