Scavenger receptor class B, type I-mediated uptake of A1AT by pulmonary endothelial cells

Angelia Lockett, Daniela Petrusca, Matthew J. Justice, Christophe Poirier, Karina A. Serban, Natalia I. Rush, Malgorzata Kamocka, Dan Predescu, Sanda Predescu, Irina Petrache

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In addition to exerting a potent anti-elastase function, α-1 antitrypsin (A1AT) maintains the structural integrity of the lung by inhibiting endothelial inflammation and apoptosis. A main serpin secreted in circulation by hepatocytes, A1AT requires uptake by the endothelium to achieve vasculoprotective effects. This active uptake mechanism, which is inhibited by cigarette smoking (CS), involves primarily clathrin-but also caveola-mediated endocytosis and may require active binding to a receptor. Because circulating A1AT binds to high-density lipoprotein (HDL), we hypothesized that scavenging receptors are candidates for endothelial uptake of the serpin. Although the low-density lipo-protein (LDL) receptor-related protein 1 (LRP1) internalizes only elastase-bound A1AT, the scavenger receptor B type I (SR-BI), which binds and internalizes HDL and is modulated by CS, may be involved in A1AT uptake. Transmission electron microscopy imaging of colloidal gold-labeled A1AT confirmed A1AT endocytosis in both clath-rin-coated vesicles and caveolae in endothelial cells. SR-BI immuno-precipitation identified binding to A1AT at the plasma membrane. Pretreatment of human lung microvascular endothelial cells with SR-B ligands (HDL or LDL), knockdown of SCARB1 expression, or neutralizing SR-BI antibodies significantly reduced A1AT uptake by 30-50%. Scarb1 null mice exhibited decreased A1AT lung content following systemic A1AT administration and reduced lung anti-inflammatory effects of A1AT supplementation during short-term CS exposure. In turn, A1AT supplementation increased lung SR-BI expression and modulated circulating lipoprotein levels in wild-type animals. These studies indicate that SR-BI is an important mediator of A1AT endocytosis in pulmonary endothelium and suggest a cross talk between A1AT and lipoprotein regulation of vascular functions.

Original languageEnglish (US)
Pages (from-to)L425-L434
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume309
Issue number4
DOIs
StatePublished - Aug 18 2015

Fingerprint

CD36 Antigens
Pancreatic Elastase
Endothelial Cells
Lung
Scavenger Receptors
HDL Lipoproteins
Endocytosis
Serpins
Caveolae
Smoking
Lipoproteins
Endothelium
Coated Vesicles
Gold Colloid
Clathrin
Proteins
Wild Animals

Keywords

  • Antiproteinase
  • Cigarette smoke
  • Lipoprotein
  • Scavenger receptor
  • Serpin

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology
  • Physiology

Cite this

Scavenger receptor class B, type I-mediated uptake of A1AT by pulmonary endothelial cells. / Lockett, Angelia; Petrusca, Daniela; Justice, Matthew J.; Poirier, Christophe; Serban, Karina A.; Rush, Natalia I.; Kamocka, Malgorzata; Predescu, Dan; Predescu, Sanda; Petrache, Irina.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 309, No. 4, 18.08.2015, p. L425-L434.

Research output: Contribution to journalArticle

Lockett, Angelia ; Petrusca, Daniela ; Justice, Matthew J. ; Poirier, Christophe ; Serban, Karina A. ; Rush, Natalia I. ; Kamocka, Malgorzata ; Predescu, Dan ; Predescu, Sanda ; Petrache, Irina. / Scavenger receptor class B, type I-mediated uptake of A1AT by pulmonary endothelial cells. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 2015 ; Vol. 309, No. 4. pp. L425-L434.
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