Scoparone exerts anti-tumor activity against DU145 prostate cancer cells via inhibition of STAT3 activity

Jeong Kook Kim, Joon Young Kim, Han Jong Kim, Keun Gyu Park, Robert Harris, Won Jea Cho, Jae Tae Lee, In Kyu Lee

Research output: Contribution to journalArticle

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Abstract

Scoparone, a natural compound isolated from Artemisia capillaris, has been used in Chinese herbal medicine to treat neonatal jaundice. Signal transducer and activator of transcription 3 (STAT3) contributes to the growth and survival of many human tumors. This study was undertaken to investigate the anti-tumor activity of scoparone against DU145 prostate cancer cells and to determine whether its effects are mediated by inhibition of STAT3 activity. Scoparone inhibited proliferation of DU145 cells via cell cycle arrest in G 1 phase. Transient transfection assays showed that scoparone repressed both constitutive and IL-6-induced transcriptional activity of STAT3. Western blot and quantitative real-time PCR analyses demonstrated that scoparone suppressed the transcription of STAT3 target genes such as cyclin D1, c-Myc, survivin, Bcl-2, and Socs3. Consistent with this, scoparone decreased phosphorylation and nuclear accumulation of STAT3, but did not reduce phosphorylation of janus kinase 2 (JAK2) or Src, the major upstream kinases responsible for STAT3 activation. Moreover, transcriptional activity of a constitutively active mutant of STAT3 (STAT3C) was inhibited by scoparone, but not by AG490, a JAK2 inhibitor. Furthermore, scoparone treatment suppressed anchorage-independent growth in soft agar and tumor growth of DU145 xenografts in nude mice, concomitant with a reduction in STAT3 phosphorylation. Computational modeling suggested that scoparone might bind the SH2 domain of STAT3. Our findings suggest that scoparone elicits an antitumor effect against DU145 prostate cancer cells in part through inhibition of STAT3 activity.

Original languageEnglish
Article numbere80391
JournalPLoS One
Volume8
Issue number11
DOIs
StatePublished - Nov 15 2013

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scoparone
STAT3 Transcription Factor
prostatic neoplasms
Tumors
Prostatic Neoplasms
transcription (genetics)
Cells
neoplasms
Neoplasms
Phosphorylation
non-specific protein-tyrosine kinase
Janus Kinase 2
phosphorylation
Growth
neoplasm cells
Artemisia capillaris
Neonatal Jaundice
Artemisia

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Scoparone exerts anti-tumor activity against DU145 prostate cancer cells via inhibition of STAT3 activity. / Kim, Jeong Kook; Kim, Joon Young; Kim, Han Jong; Park, Keun Gyu; Harris, Robert; Cho, Won Jea; Lee, Jae Tae; Lee, In Kyu.

In: PLoS One, Vol. 8, No. 11, e80391, 15.11.2013.

Research output: Contribution to journalArticle

Kim, Jeong Kook ; Kim, Joon Young ; Kim, Han Jong ; Park, Keun Gyu ; Harris, Robert ; Cho, Won Jea ; Lee, Jae Tae ; Lee, In Kyu. / Scoparone exerts anti-tumor activity against DU145 prostate cancer cells via inhibition of STAT3 activity. In: PLoS One. 2013 ; Vol. 8, No. 11.
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