Screening and identification of phage antibodies from phage display library against Schistosoma japonicum

Dai xiong Chen, Mu hua Yu, Zhi gang Lei, Bin Zheng, A. He, Rui lin Zhang, Xi mei Zhan

Research output: Contribution to journalArticle

Abstract

OBJECTIVE: To obtain single chain variable fragment(ScFv) against the circulating antigen(CAg) from Schistosoma japonicum(Sj). METHODS: Metabolic antigen of adult worm of Sj (Sj-MAg) was used in the panning of phage library against Schistosoma japonicum. The activity of Sj-MAg-binding phage clones was assayed by ELISA. The specificity of expression products of the positive clones was analyzed by ELISA, SDS-PAGE and Western blotting. RESULTS: Seventy-two randomly selected clones were tested for the presence of anti-Sj-MAg ScFvs, 6 clones showed positive. The specificity of these 6 clones was confirmed by binding them to antigens of other four trematodes. Two clones (B04, C24) were found to bind to Sj-MAg but not to any of the antigens of other four trematodes and their expression products were about 31 kDa in size. CONCLUSION: ScFv antibodies against the circulating antigens from Schistosoma japonicum Sj-MAg can be selected and manufactured from the antibody library.

Original languageEnglish (US)
Pages (from-to)261-263
Number of pages3
JournalZhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases
Volume21
Issue number5
StatePublished - 2003

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ASJC Scopus subject areas

  • Medicine(all)

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