Screening for the effects of antiepileptic drugs on short term plasticity using a time efficient bioassay

Ghassan I. Gholmieh, Spiros H. Courellis, Lan S. Chen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Screening for changes in the short-term plasticity (STP) characteristics induced by antiepileptic drugs (AEDs) can be accelerated using a novel in vitro bioassay. The bioassay is based on the analysis of varying population spike (PS) amplitudes recorded in the CA1 region of the hippocampal slice in response to Poisson distributed random electrical stimuli. Three antiepileptic drugs (phenytoin 100 μM, carbamazepine 100 μM, and valproate 700 μM) were tested at maximal effective therapeutic concentrations. The data were analyzed using an advanced nonlinear approach that is more specific and time-efficient than the conventional paired pulse and fixed frequency train methods. STP was quantified by the first and the second order Volterra kernels. The first order kernel (k1) represented the mean PS amplitude while the second order kernel (k2) quantified the effect on the current PS amplitude of the interaction between the current stimulus impulse and each past stimulus impulse within a time (memory) window μ. The mean PS (k1) decreased by 15%, 10%, and 7% when phenytoin, carbamazepine, and valproate were added respectively. Phenytoin caused an increase in the k2 peak facilitation in the high frequency domain. Carbamazepine impaired frequency facilitation in the theta frequency range by causing a left shift in the second order kernel.

Original languageEnglish (US)
Title of host publication29th Annual International Conference of IEEE-EMBS, Engineering in Medicine and Biology Society, EMBC'07
Pages2247-2252
Number of pages6
DOIs
StatePublished - Dec 1 2007
Event29th Annual International Conference of IEEE-EMBS, Engineering in Medicine and Biology Society, EMBC'07 - Lyon, France
Duration: Aug 23 2007Aug 26 2007

Publication series

NameAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
ISSN (Print)0589-1019

Other

Other29th Annual International Conference of IEEE-EMBS, Engineering in Medicine and Biology Society, EMBC'07
CountryFrance
CityLyon
Period8/23/078/26/07

ASJC Scopus subject areas

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

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  • Cite this

    Gholmieh, G. I., Courellis, S. H., & Chen, L. S. (2007). Screening for the effects of antiepileptic drugs on short term plasticity using a time efficient bioassay. In 29th Annual International Conference of IEEE-EMBS, Engineering in Medicine and Biology Society, EMBC'07 (pp. 2247-2252). [4352772] (Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings). https://doi.org/10.1109/IEMBS.2007.4352772