Screening for the effects of antiepileptic drugs on short term plasticity using a time efficient bioassay

Ghassan I. Gholmieh, Spiros H. Courellis, Lan Chen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Screening for changes in the short-term plasticity (STP) characteristics induced by antiepileptic drugs (AEDs) can be accelerated using a novel in vitro bioassay. The bioassay is based on the analysis of varying population spike (PS) amplitudes recorded in the CA1 region of the hippocampal slice in response to Poisson distributed random electrical stimuli. Three antiepileptic drugs (phenytoin 100 μM, carbamazepine 100 μM, and valproate 700 μM) were tested at maximal effective therapeutic concentrations. The data were analyzed using an advanced nonlinear approach that is more specific and time-efficient than the conventional paired pulse and fixed frequency train methods. STP was quantified by the first and the second order Volterra kernels. The first order kernel (k1) represented the mean PS amplitude while the second order kernel (k2) quantified the effect on the current PS amplitude of the interaction between the current stimulus impulse and each past stimulus impulse within a time (memory) window μ. The mean PS (k1) decreased by 15%, 10%, and 7% when phenytoin, carbamazepine, and valproate were added respectively. Phenytoin caused an increase in the k2 peak facilitation in the high frequency domain. Carbamazepine impaired frequency facilitation in the theta frequency range by causing a left shift in the second order kernel.

Original languageEnglish (US)
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Pages2247-2252
Number of pages6
DOIs
StatePublished - 2007
Externally publishedYes
Event29th Annual International Conference of IEEE-EMBS, Engineering in Medicine and Biology Society, EMBC'07 - Lyon, France
Duration: Aug 23 2007Aug 26 2007

Other

Other29th Annual International Conference of IEEE-EMBS, Engineering in Medicine and Biology Society, EMBC'07
CountryFrance
CityLyon
Period8/23/078/26/07

Fingerprint

Bioassay
Plasticity
Screening
Data storage equipment

ASJC Scopus subject areas

  • Biomedical Engineering

Cite this

Gholmieh, G. I., Courellis, S. H., & Chen, L. (2007). Screening for the effects of antiepileptic drugs on short term plasticity using a time efficient bioassay. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (pp. 2247-2252). [4352772] https://doi.org/10.1109/IEMBS.2007.4352772

Screening for the effects of antiepileptic drugs on short term plasticity using a time efficient bioassay. / Gholmieh, Ghassan I.; Courellis, Spiros H.; Chen, Lan.

Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. 2007. p. 2247-2252 4352772.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Gholmieh, GI, Courellis, SH & Chen, L 2007, Screening for the effects of antiepileptic drugs on short term plasticity using a time efficient bioassay. in Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings., 4352772, pp. 2247-2252, 29th Annual International Conference of IEEE-EMBS, Engineering in Medicine and Biology Society, EMBC'07, Lyon, France, 8/23/07. https://doi.org/10.1109/IEMBS.2007.4352772
Gholmieh GI, Courellis SH, Chen L. Screening for the effects of antiepileptic drugs on short term plasticity using a time efficient bioassay. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. 2007. p. 2247-2252. 4352772 https://doi.org/10.1109/IEMBS.2007.4352772
Gholmieh, Ghassan I. ; Courellis, Spiros H. ; Chen, Lan. / Screening for the effects of antiepileptic drugs on short term plasticity using a time efficient bioassay. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. 2007. pp. 2247-2252
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