SDF-1 suppresses cytokine-induced adhesion of human haemopoietic progenitor cells to immobilized fibronectin

Akihiko Gotoh, Suzanna Reid, Keisuke Miyazawa, Hal E. Broxmeyer

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

The process of haemopoietic cell homing to the microenvironment includes migration and adhesion. SDF-1 is a C-X-C chemokine that acts as a chemoattractant for haemopoietic progenitors. Adhesion of haemopoietic progenitors to immobilized fibronectin is up-regulated by stimulation with cytokines. We assessed the effects of SDF-1 on cytokine-induced adhesion of progenitor cells to fibronectin. In factor-dependent human MO7e cells and primary CD34+ cord blood cells, treatment with SDF-1 dose-dependently suppressed cytokine-induced adhesion. Pretreatment with pertussis toxin reversed adhesion-inhibition, suggesting that activation of G-coupled proteins are involved in the intracellular signalling of this process. These data suggest that SDF-1 not only acts as a chemoattractant but also participates in modulating adhesiveness of haemopoietic progenitors to extracellular matrix components.

Original languageEnglish (US)
Pages (from-to)171-174
Number of pages4
JournalBritish journal of haematology
Volume106
Issue number1
DOIs
StatePublished - Aug 2 1999

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Keywords

  • Cell adhesion
  • Chemokine
  • Fibronectin
  • SDF-1
  • Steel factor
  • Thrombopoietin

ASJC Scopus subject areas

  • Hematology

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