SDF-1/CXCL12 enhances in vitro replating capacity of murine and human multipotential and macrophage progenitor cells

Hal E. Broxmeyer, Jorge Alejandro Henao Mejia, Giao Hangoc, Cecilia Barese, Mary Dinauer, Scott Cooper

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Hematopoietic progenitor cells (HPCs) manifest a limited self-renewal capacity, as determined by a surrogate assay involving replating capacity of single colonies in vitro with generation of secondary colonies. Stromal cell-derived factor-1 (SDF-1/CXCL12), has been implicated in regulation of hematopoiesis through its modulation of hematopoietic stem cell (HSC) and HPC migration, homing, mobilization, and survival. We used bone marrow cells from SDF-1/CXCL12 transgenic and littermate control mice, and culture of normal mouse bone marrow and human cord blood cells plated in the presence or absence of recombinant SDF-1/CXCL12 to evaluate a role for SDF-1/CXCL12 in the replating capability in vitro of multipotential [colony-forming units (CFU)-GEMM] and macrophage (CFU-M) progenitor cells. Competitive repopulating capacity of mouse HSCs was assessed in lethally irradiated mice. Transgenic or exogenous SDF-1/CXCL12 significantly enhanced numbers of secondary colonies formed from primary CFU-GEMM or CFU-M colonies. In the limited setting of our in vivo studies, the SDF-1/CXCL12 transgene did not influence HSC competitive repopulation. However, the results suggest that SDF-1/CXCL12 enhances in vitro replating/self-renewal of HPCs, which may contribute to myelopoiesis in vivo. This information may be of value to ex vivo expansion of HPCs/HSCs.

Original languageEnglish (US)
Pages (from-to)589-596
Number of pages8
JournalStem cells and development
Volume16
Issue number4
DOIs
StatePublished - Aug 2007

ASJC Scopus subject areas

  • Hematology
  • Developmental Biology
  • Cell Biology

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