SDF-1/CXCL12 modulates mitochondrial respiration of immature blood cells in a bi-phasic manner

Steven Messina-Graham, Hal Broxmeyer

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

SDF-1/CXCL12 is a potent chemokine required for the homing and engraftment of hematopoietic stem and progenitor cells. Previous data from our group has shown that in an SDF-1/CXCL12 transgenic mouse model, lineage- Sca-1+ c-Kit+ (LSK) bone marrow cells have reduced mitochondrial membrane potential versus wild-type. These results suggested that SDF-1/CXCL12 may function to keep mitochondrial respiration low in immature blood cells in the bone marrow. Low mitochondrial metabolism helps to maintain low levels of reactive oxygen species (ROS), which can influence differentiation. To test whether SDF-1/CXCL12 regulates mitochondrial metabolism, we employed the human leukemia cell line HL-60, that expresses high levels of the SDF-1/CXCL12 receptor, CXCR4, as a model of hematopoietic progenitor cells in vitro. We treated HL-60 cells with SDF-1/CXCL12 for 2 and 24 h. Oxygen consumption rates (OCR), mitochondrial-associated ATP production, mitochondrial mass, and mitochondrial membrane potential of HL-60 cells were significantly reduced at 2 h and increased at 24 h as compared to untreated control cells. These biphasic effects of SDF-1/CXCL12 were reproduced with lineage negative primary mouse bone marrow cells, suggesting a novel function of SDF-1/CXCL12 in modulating mitochondrial respiration by regulating mitochondrial oxidative phosphorylation, ATP production and mitochondrial content.

Original languageEnglish (US)
Pages (from-to)13-18
Number of pages6
JournalBlood Cells, Molecules and Diseases
Volume58
DOIs
StatePublished - May 1 2016

Fingerprint

Hematopoietic Stem Cells
Blood Cells
Respiration
Mitochondrial Membrane Potential
HL-60 Cells
Bone Marrow Cells
Adenosine Triphosphate
CXCR4 Receptors
Oxidative Phosphorylation
Chemokines
Oxygen Consumption
Transgenic Mice
Reactive Oxygen Species
Leukemia
Bone Marrow
Cell Line

Keywords

  • Blood cells
  • Mitochondria
  • Oxygen consumption
  • SDF-1/CXCL12

ASJC Scopus subject areas

  • Molecular Biology
  • Molecular Medicine
  • Hematology
  • Cell Biology

Cite this

SDF-1/CXCL12 modulates mitochondrial respiration of immature blood cells in a bi-phasic manner. / Messina-Graham, Steven; Broxmeyer, Hal.

In: Blood Cells, Molecules and Diseases, Vol. 58, 01.05.2016, p. 13-18.

Research output: Contribution to journalArticle

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