SDS Interferes with SaeS Signaling of Staphylococcus aureus Independently of SaePQ

Phuti E. Makgotlho, Gabriella Marincola, Daniel Schäfer, Qian Liu, Taeok Bae, Tobias Geiger, Elizabeth Wasserman, Christiane Wolz, Wilma Ziebuhr, Bhanu Sinha

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The Staphylococcus aureus regulatory saePQRS system controls the expression of numerous virulence factors, including extracellular adherence protein (Eap), which amongst others facilitates invasion of host cells. The saePQRS operon codes for 4 proteins: the histidine kinase SaeS, the response regulator SaeR, the lipoprotein SaeP and the transmembrane protein SaeQ. S. aureus strain Newman has a single amino acid substitution in the transmembrane domain of SaeS (L18P) which results in constitutive kinase activity. SDS was shown to be one of the signals interfering with SaeS activity leading to inhibition of the sae target gene eap in strains with SaeSL but causing activation in strains containing SaeSP. Here, we analyzed the possible involvement of the SaeP protein and saePQ region in SDS-mediated sae/eap expression. We found that SaePQ is not needed for SDS-mediated SaeS signaling. Furthermore, we could show that SaeS activity is closely linked to the expression of Eap and the capacity to invade host cells in a number of clinical isolates. This suggests that SaeS activity might be directly modulated by structurally non-complex environmental signals, as SDS, which possibly altering its kinase/phosphatase activity.

Original languageEnglish (US)
Article numbere71644
JournalPloS one
Volume8
Issue number8
DOIs
StatePublished - Aug 20 2013

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Makgotlho, P. E., Marincola, G., Schäfer, D., Liu, Q., Bae, T., Geiger, T., Wasserman, E., Wolz, C., Ziebuhr, W., & Sinha, B. (2013). SDS Interferes with SaeS Signaling of Staphylococcus aureus Independently of SaePQ. PloS one, 8(8), [e71644]. https://doi.org/10.1371/journal.pone.0071644