Searching for hemostatic modifier genes affecting the phenotype of mice with very low levels of FVII

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Abstract

FVIItTA/- mice are heterozygous for the FVII- null allele and a gene-targeted allele expressing very low levels of FVII. Approximately half the FVIItTA/- in a mixed C57Bl/6:129x1/SVJ background survive much longer than mice in a C57Bl/6 background, suggesting that genetic background affects the survival of mice expressing very low levels of FVII. A lineage of long-term surviving FVIItTA/- mice in a predominantly C57Bl/6 background was generated by selecting 16 weeks or older FVIItTA/- males and breeding them with C57Bl/6 FVII+/- females. It is postulated that these mice maintain 129x1/SVJ alleles at putative hemostatic modifier loci necessary for the survival of mice expressing very low levels of FVII. A SNP genomic scan was performed on DNA from long-term surviving FVIItTA/- mice. The scan identified 8 regions enriched for 129x1/SVJ sequences that might contain putative hemostatic modifier genes.

Original languageEnglish
Pages (from-to)131-134
Number of pages4
JournalBlood Cells, Molecules and Diseases
Volume36
Issue number2
DOIs
StatePublished - Mar 2006

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Modifier Genes
Hemostatics
Phenotype
Alleles
Breeding
Single Nucleotide Polymorphism
DNA

Keywords

  • Factor VII
  • Hemostasis
  • Modifier genes
  • Quantitative traits
  • SNPs
  • Strain differences

ASJC Scopus subject areas

  • Molecular Biology
  • Molecular Medicine
  • Hematology

Cite this

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title = "Searching for hemostatic modifier genes affecting the phenotype of mice with very low levels of FVII",
abstract = "FVIItTA/- mice are heterozygous for the FVII- null allele and a gene-targeted allele expressing very low levels of FVII. Approximately half the FVIItTA/- in a mixed C57Bl/6:129x1/SVJ background survive much longer than mice in a C57Bl/6 background, suggesting that genetic background affects the survival of mice expressing very low levels of FVII. A lineage of long-term surviving FVIItTA/- mice in a predominantly C57Bl/6 background was generated by selecting 16 weeks or older FVIItTA/- males and breeding them with C57Bl/6 FVII+/- females. It is postulated that these mice maintain 129x1/SVJ alleles at putative hemostatic modifier loci necessary for the survival of mice expressing very low levels of FVII. A SNP genomic scan was performed on DNA from long-term surviving FVIItTA/- mice. The scan identified 8 regions enriched for 129x1/SVJ sequences that might contain putative hemostatic modifier genes.",
keywords = "Factor VII, Hemostasis, Modifier genes, Quantitative traits, SNPs, Strain differences",
author = "Elliot Rosen and Xiaoling Xuei and Mark Suckow and Howard Edenberg",
year = "2006",
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language = "English",
volume = "36",
pages = "131--134",
journal = "Blood Cells, Molecules, and Diseases",
issn = "1079-9796",
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T1 - Searching for hemostatic modifier genes affecting the phenotype of mice with very low levels of FVII

AU - Rosen, Elliot

AU - Xuei, Xiaoling

AU - Suckow, Mark

AU - Edenberg, Howard

PY - 2006/3

Y1 - 2006/3

N2 - FVIItTA/- mice are heterozygous for the FVII- null allele and a gene-targeted allele expressing very low levels of FVII. Approximately half the FVIItTA/- in a mixed C57Bl/6:129x1/SVJ background survive much longer than mice in a C57Bl/6 background, suggesting that genetic background affects the survival of mice expressing very low levels of FVII. A lineage of long-term surviving FVIItTA/- mice in a predominantly C57Bl/6 background was generated by selecting 16 weeks or older FVIItTA/- males and breeding them with C57Bl/6 FVII+/- females. It is postulated that these mice maintain 129x1/SVJ alleles at putative hemostatic modifier loci necessary for the survival of mice expressing very low levels of FVII. A SNP genomic scan was performed on DNA from long-term surviving FVIItTA/- mice. The scan identified 8 regions enriched for 129x1/SVJ sequences that might contain putative hemostatic modifier genes.

AB - FVIItTA/- mice are heterozygous for the FVII- null allele and a gene-targeted allele expressing very low levels of FVII. Approximately half the FVIItTA/- in a mixed C57Bl/6:129x1/SVJ background survive much longer than mice in a C57Bl/6 background, suggesting that genetic background affects the survival of mice expressing very low levels of FVII. A lineage of long-term surviving FVIItTA/- mice in a predominantly C57Bl/6 background was generated by selecting 16 weeks or older FVIItTA/- males and breeding them with C57Bl/6 FVII+/- females. It is postulated that these mice maintain 129x1/SVJ alleles at putative hemostatic modifier loci necessary for the survival of mice expressing very low levels of FVII. A SNP genomic scan was performed on DNA from long-term surviving FVIItTA/- mice. The scan identified 8 regions enriched for 129x1/SVJ sequences that might contain putative hemostatic modifier genes.

KW - Factor VII

KW - Hemostasis

KW - Modifier genes

KW - Quantitative traits

KW - SNPs

KW - Strain differences

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U2 - 10.1016/j.bcmd.2005.12.037

DO - 10.1016/j.bcmd.2005.12.037

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JO - Blood Cells, Molecules, and Diseases

JF - Blood Cells, Molecules, and Diseases

SN - 1079-9796

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