Secretion of insulin-like growth factor-I (IGF-I) and IGF-binding proteins from bovine mammary tissue in vitro

P. G. Campbell, Todd Skaar, J. R. Vega, C. R. Baumrucker

Research output: Contribution to journalArticle

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Abstract

In vitro, insulin-like growth factor-I (IGF-I) promotes both growth and development of bovine mammary tissue. In vivo, the effects of IGF-I may encompass endocrine, paracrine or autocrine mediation. We addressed the possibility of paracrine/autocrine effects of IGF-I in the mammary gland by examining the in-vitro secretion of IGF-I and IGF-binding proteins (IGFBPs) from bovine mammary tissue. Bovine mammary explants from pregnant non-lactating and lactating non-pregnant animals were found to synthesize and secrete IGF-I and IGFBPs. Mammary acini cultures, representative of mammary secretory epithelia, secreted both IGF-I and IGFBP, but synthesized only IGFBP. Concentrations of IGF-I in conditioned media from explants were 1.54 and 0.72 fmol/μg DNA for pregnant and lactating animals respectively. Concentrations of IGFBPs in conditioned media from explants were similar for both physiological states at 2529 pmol 125I-labelled IGF-I bound/μg DNA. Ligand/Western blotting procedures identified four IGFBPs of 29, 33, 37 and 44 kDa for acini cultures and five IGFBPs of 28, 31, 36, 44 and 46 kDa for explant cultures. Similar affinities for IGF-I and IGF-II were shown by IGFBP, using 125I-labelled recombinant human IGF-I as the competing ligand (median effective dose (ED50) of 0.085 pmol). When 125I-labelled bovine IGF-II was used as the ligand, only bovine IGF-II (ED50 of 0.25 pmol) inhibited binding. The addition of prolactin, insulin and cortisol, with or without GH, did not affect secretion of either IGF-I or IGFBP. This report describes the ability of normal mammary tissue to synthesize and secrete IGF-I and IGFBPs.

Original languageEnglish (US)
Pages (from-to)219-228
Number of pages10
JournalJournal of Endocrinology
Volume128
Issue number2
StatePublished - 1991
Externally publishedYes

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Insulin-Like Growth Factor Binding Proteins
Insulin-Like Growth Factor I
Breast
Insulin-Like Growth Factor II
Conditioned Culture Medium
Ligands
In Vitro Techniques
DNA
Human Mammary Glands
Growth and Development
Prolactin
Hydrocortisone
Epithelium
Western Blotting

ASJC Scopus subject areas

  • Endocrinology

Cite this

Secretion of insulin-like growth factor-I (IGF-I) and IGF-binding proteins from bovine mammary tissue in vitro. / Campbell, P. G.; Skaar, Todd; Vega, J. R.; Baumrucker, C. R.

In: Journal of Endocrinology, Vol. 128, No. 2, 1991, p. 219-228.

Research output: Contribution to journalArticle

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