Segmentation, reconstruction, and analysis of blood thrombi in 2-photon microscopy images

Jian Mu, Xiaomin Liu, Malgorzata Kamocka, Zhiliang Xu, Mark S. Alber, Elliot Rosen, Danny Z. Chen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

6 Citations (Scopus)

Abstract

In this paper, we study the problem of segmenting, reconstructing, and analyzing the structure and growth of thrombi (clots) in vivo in blood vessels based on 2-photon microscopic image data. First, we develop an algorithm for segmenting clots in 3-D microscopic images which incorporates the density-based clustering algorithm and other methods for dealing with imaging artifacts. Next, we apply the union-of-balls (or alpha-shape) algorithm to reconstruct the boundary of clots in 3-D. Finally, we perform experimental analysis on the reconstructed clots and obtain quantitative data of thrombus growth and structures. The experiments are conducted on laser-induced injuries in vessels of two types of mice (the wild type and the type with low levels of coagulation factor VII). By analyzing and comparing the developing clot structures based on their reconstruction from image data, we obtain results of biomedical significance. Our quantitative analysis of the clot composition leads to better understanding of the thrombus development, which is also valuable to the modeling and verification of computational simulation of thrombogenesis.

Original languageEnglish
Title of host publicationProceedings - IEEE Symposium on Computer-Based Medical Systems
DOIs
StatePublished - 2009
Event2009 22nd IEEE International Symposium on Computer-Based Medical Systems, CBMS 2009 - Albuquerque, NM, United States
Duration: Aug 2 2009Aug 5 2009

Other

Other2009 22nd IEEE International Symposium on Computer-Based Medical Systems, CBMS 2009
CountryUnited States
CityAlbuquerque, NM
Period8/2/098/5/09

Fingerprint

Photons
Microscopy
Microscopic examination
Thrombosis
Blood
Blood vessels
Coagulation
Chemical analysis
Clustering algorithms
Factor VII
Computer-Assisted Image Processing
Three-Dimensional Imaging
Growth
Imaging techniques
Artifacts
Blood Vessels
Cluster Analysis
Lasers
Experiments
Wounds and Injuries

ASJC Scopus subject areas

  • Computer Science Applications
  • Radiology Nuclear Medicine and imaging

Cite this

Mu, J., Liu, X., Kamocka, M., Xu, Z., Alber, M. S., Rosen, E., & Chen, D. Z. (2009). Segmentation, reconstruction, and analysis of blood thrombi in 2-photon microscopy images. In Proceedings - IEEE Symposium on Computer-Based Medical Systems [5255347] https://doi.org/10.1109/CBMS.2009.5255347

Segmentation, reconstruction, and analysis of blood thrombi in 2-photon microscopy images. / Mu, Jian; Liu, Xiaomin; Kamocka, Malgorzata; Xu, Zhiliang; Alber, Mark S.; Rosen, Elliot; Chen, Danny Z.

Proceedings - IEEE Symposium on Computer-Based Medical Systems. 2009. 5255347.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Mu, J, Liu, X, Kamocka, M, Xu, Z, Alber, MS, Rosen, E & Chen, DZ 2009, Segmentation, reconstruction, and analysis of blood thrombi in 2-photon microscopy images. in Proceedings - IEEE Symposium on Computer-Based Medical Systems., 5255347, 2009 22nd IEEE International Symposium on Computer-Based Medical Systems, CBMS 2009, Albuquerque, NM, United States, 8/2/09. https://doi.org/10.1109/CBMS.2009.5255347
Mu J, Liu X, Kamocka M, Xu Z, Alber MS, Rosen E et al. Segmentation, reconstruction, and analysis of blood thrombi in 2-photon microscopy images. In Proceedings - IEEE Symposium on Computer-Based Medical Systems. 2009. 5255347 https://doi.org/10.1109/CBMS.2009.5255347
Mu, Jian ; Liu, Xiaomin ; Kamocka, Malgorzata ; Xu, Zhiliang ; Alber, Mark S. ; Rosen, Elliot ; Chen, Danny Z. / Segmentation, reconstruction, and analysis of blood thrombi in 2-photon microscopy images. Proceedings - IEEE Symposium on Computer-Based Medical Systems. 2009.
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