Segmentation, reconstruction, and analysis of blood thrombi in 2-photon microscopy images

Jian Mu, Xiaomin Liu, Malgorzata M. Kamocka, Zhiliang Xu, Mark S. Alber, Elliot D. Rosen, Danny Z. Chen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

6 Scopus citations

Abstract

In this paper, we study the problem of segmenting, reconstructing, and analyzing the structure and growth of thrombi (clots) in vivo in blood vessels based on 2-photon microscopic image data. First, we develop an algorithm for segmenting clots in 3-D microscopic images which incorporates the density-based clustering algorithm and other methods for dealing with imaging artifacts. Next, we apply the union-of-balls (or alpha-shape) algorithm to reconstruct the boundary of clots in 3-D. Finally, we perform experimental analysis on the reconstructed clots and obtain quantitative data of thrombus growth and structures. The experiments are conducted on laser-induced injuries in vessels of two types of mice (the wild type and the type with low levels of coagulation factor VII). By analyzing and comparing the developing clot structures based on their reconstruction from image data, we obtain results of biomedical significance. Our quantitative analysis of the clot composition leads to better understanding of the thrombus development, which is also valuable to the modeling and verification of computational simulation of thrombogenesis.

Original languageEnglish (US)
Title of host publication2009 22nd IEEE International Symposium on Computer-Based Medical Systems, CBMS 2009
DOIs
StatePublished - Nov 23 2009
Event2009 22nd IEEE International Symposium on Computer-Based Medical Systems, CBMS 2009 - Albuquerque, NM, United States
Duration: Aug 2 2009Aug 5 2009

Publication series

NameProceedings - IEEE Symposium on Computer-Based Medical Systems
ISSN (Print)1063-7125

Other

Other2009 22nd IEEE International Symposium on Computer-Based Medical Systems, CBMS 2009
CountryUnited States
CityAlbuquerque, NM
Period8/2/098/5/09

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Computer Science Applications

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    Mu, J., Liu, X., Kamocka, M. M., Xu, Z., Alber, M. S., Rosen, E. D., & Chen, D. Z. (2009). Segmentation, reconstruction, and analysis of blood thrombi in 2-photon microscopy images. In 2009 22nd IEEE International Symposium on Computer-Based Medical Systems, CBMS 2009 [5255347] (Proceedings - IEEE Symposium on Computer-Based Medical Systems). https://doi.org/10.1109/CBMS.2009.5255347