Selection of DNA ligands for protein kinase C-δ

Prabodhika Mallikaratchy, Robert Stahelin, Zehui Cao, Wonhwa Cho, Weihong Tan

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Protein kinase Cs are a family of serine and threonine kinases that mediate a wide variety of cellular signalling processes such as cell growth, differentiation, apoptosis and tumor development. We have selected high-affinity DNA aptamers for PKCδ by capillary electrophoresis based SELEX (systematic evolution of ligands by exponential enrichment, CE-SELEX). We have demonstrated that fluorescently tagged PB9 aptamer can specifically recognize PKCδ under in vitro conditions. The Kd of the aptamer-protein binding is 122 nM. These aptamers will enable us to apply fluorescently labelled probes to study the spatiotemporal dynamics and activation of individual endogenous PKC isoforms during various cell signalling processes.

Original languageEnglish (US)
Pages (from-to)3229-3231
Number of pages3
JournalChemical Communications
Issue number30
DOIs
StatePublished - 2006
Externally publishedYes

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Cell signaling
Protein Kinase C
DNA
Ligands
Nucleotide Aptamers
Proteins
Capillary electrophoresis
Protein-Serine-Threonine Kinases
Cell growth
Cell death
Protein Kinases
Tumors
Protein Isoforms
Chemical activation
Apoptosis
Protein Binding

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Selection of DNA ligands for protein kinase C-δ. / Mallikaratchy, Prabodhika; Stahelin, Robert; Cao, Zehui; Cho, Wonhwa; Tan, Weihong.

In: Chemical Communications, No. 30, 2006, p. 3229-3231.

Research output: Contribution to journalArticle

Mallikaratchy, Prabodhika ; Stahelin, Robert ; Cao, Zehui ; Cho, Wonhwa ; Tan, Weihong. / Selection of DNA ligands for protein kinase C-δ. In: Chemical Communications. 2006 ; No. 30. pp. 3229-3231.
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