Selective identification of Vα14i T cells using slide-immobilized, CD1d-antigen complexes

Alfred J. Zullo, Randy R. Brutkiewicz, Elizabeth J. Taparowsky

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

The ability to correlate changes in antigen-reactive lymphocytes with disease will provide information needed to develop strategies for combating illness. One critical group of lymphocytes are the CD1-restricted T cells. It is desirable to use CD1 molecules in an array format to query CD1-restricted lymphocytes in humans. To investigate the feasibility of this technique, we employed mCD1d and α-galactosylceramide to demonstrate that-slide immobilized, CD1d-α-GalCer complexes capture an NKT cell hybridoma in the presence of a competitor. The success of this scheme represents the first step toward the development of CD1-antigen arrays that could be used to profile biological samples.

Original languageEnglish (US)
Pages (from-to)207-212
Number of pages6
JournalJournal of Immunoassay and Immunochemistry
Volume27
Issue number3
DOIs
StatePublished - Sep 1 2006

Keywords

  • Antigen
  • Array
  • CD1
  • Glycolipid
  • Hybridoma
  • NKT cell

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

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