Selective inhibition of vasoconstrictor responses by platelet-activating factor in rat kidney

Rajash Handa, Jack W. Strandhoy, Vardaman M. Buckalew

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We examined the effect of platelet-activating factor (PAF) on renal vascular reactivity in the pentobarbital sodium-anesthetized male Wistar rat. Intrarenal infusion of C16-PAF at hypotensive (2.5 ng · min-1 · kg-1) or nonhypotensive (0.5 ng · min-1 · kg-1) doses caused renal vasodilation and dose dependently antagonized the renal vasoconstrictor responses of intrarenal boluses of angiotensin II (ANG II) > norepinephrine (NE) > vasopressin (AVP). PAF infusion at the high dose did not alter non-receptor-mediated renal vasoconstriction induced by intrarenal KCl injection. The inhibitory effect of PAF on agonist-induced renal vasoconstriction was accentuated by eicosanoid synthesis inhibition (indomethacin or dexamethasone), unaffected by dopamine-receptor blockade (haloperidol) but was totally abolished by PAF receptor antagonism (L-659,989). In contrast, intrarenal infusion of a calcium channel antagonist (nimodipine) or an intracellular calcium channel antagonist (TMB-8) equally inhibited the renal vasoconstrictor responses of ANG II, NE, and AVP. Thus PAF can cause renal vasodilation in the rat kidney and dose-dependently antagonizes the renal vasoconstrictor responses of ANG II > NE > AVP. The inhibitory effect of PAF on renal vasoconstrictor responses is mediated by PAF receptors and does not appear to be due to a nonspecific membrane effect, reduction in calcium mobilization, or the release of vasodilatory eicosanoids or dopamine.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume261
Issue number1 30-1
StatePublished - 1991
Externally publishedYes

Fingerprint

Platelet Activating Factor
Vasoconstrictor Agents
Kidney
Angiotensin II
Norepinephrine
Eicosanoids
Calcium Channel Blockers
Vasoconstriction
Vasodilation
L 659989
Nimodipine
Dopamine Receptors
Haloperidol
Pentobarbital
Vasopressins
Indomethacin
Dexamethasone
Blood Vessels
Wistar Rats
Dopamine

Keywords

  • Angiotensin II
  • Blood pressure
  • Dopamine
  • Eicosanoids
  • Nimodipine
  • Norepinephrine
  • Platelet-activating factor receptor antagonist
  • Renal blood flow
  • TMB-8
  • Vasodilation
  • Vasopressin

ASJC Scopus subject areas

  • Physiology

Cite this

Selective inhibition of vasoconstrictor responses by platelet-activating factor in rat kidney. / Handa, Rajash; Strandhoy, Jack W.; Buckalew, Vardaman M.

In: American Journal of Physiology - Renal Fluid and Electrolyte Physiology, Vol. 261, No. 1 30-1, 1991.

Research output: Contribution to journalArticle

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