Selective maintenance of cultured epithelial cells from DMBA-induced mammary tumours by bovine colostrum supplement

M. T. Tseng, A. R. Safa, R. Ballou

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Growth kinetics in cultures of DMBA-induced mammary tumours supplemented with bovine colostrum were compared with the kinetics of cultures maintained with the conventional supplement of foetal calf serum. Although the latter permitted a greater degree of cell proliferation, a substantial amount of the cell growth was due to the fibroblastic proliferation. In the presence of bovine colostrum, epithelial islands surrounded by a few solitary cells became established. Unlike the foetal calf serum supplemented cultures, these cultures frequently did not become completely confluent within 7 days. The absence of fibroblasts in colostrum supplemented cultures was confirmed by electron microscopy. Results from this study suggest that colostrum may be useful in selective maintenance of primary cultures of epithelial origin.

Original languageEnglish (US)
Pages (from-to)85-92
Number of pages8
JournalCell and Tissue Kinetics
Volume16
Issue number1
StatePublished - Jan 1 1983
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology

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