Self-complementary adeno-associated virus 2 (AAV)-T cell protein tyrosine phosphatase vectors as helper viruses to Improve transduction efficiency of conventional single-stranded AAV vectors in vitro and in vivo

Li Zhong, Linyuan Chen, Yanjun Li, Keyun Qing, Kirsten A. Weigel-Kelley, Rebecca Chan, Mervin Yoder, Arun Srivastava

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Recombinant vectors based on adeno-associated virus type 2 (AAV) target the liver efficiently, but the transgene expression is limited to ∼5% of hepatocytes. The lack of efficient transduction is due, in part, to the presence of a cellular protein, FKBP52, phosphorylated forms of which inhibit the viral second-strand DNA synthesis. We have documented that dephosphorylation of FKBP52 at tyrosine residues by the cellular T cell protein tyrosine phosphatase (TC-PTP) enhances AAV-mediated transduction in primary murine hematopoietic cells from TC-PTP-transgenic mice. We have also documented that AAV-mediated transduction is significantly enhanced in hepatocytes in TC-PTP-transgenic as well as in FKBP52-deficient mice because of efficient viral second-strand DNA synthesis. In this study, we evaluated whether co-infection of conventional single-stranded AAV vectors with self-complementary AAV-TC-PTP vectors leads to increased transduction efficiency of conventional AAV vectors in established human cell lines in vitro and in primary murine hepatocytes in vivo. We demonstrate here that scAAV-TC-PTP vectors serve as a helper virus in augmenting the transduction efficiency of conventional AAV vectors in vitro as well as in vivo which correlates directly with the extent of second-strand DNA synthesis of conventional single-stranded AAV vectors. Toxicological studies following tail-vein injections of scAAV-TC-PTP vectors in experimental mice show no evidence of any adverse effect in any of the organs in any of the mice for up to 13 weeks. Thus, this novel co-infection strategy should be useful in circumventing one of the major obstacles in the optimal use of recombinant AAV vectors in human gene therapy.

Original languageEnglish
Pages (from-to)950-957
Number of pages8
JournalMolecular Therapy
Volume10
Issue number5
DOIs
StatePublished - Nov 2004

Fingerprint

Non-Receptor Type 2 Protein Tyrosine Phosphatase
Helper Viruses
Dependovirus
Hepatocytes
Coinfection
DNA
In Vitro Techniques
Transgenes
Genetic Therapy
Toxicology
Transgenic Mice
Tyrosine
Tail
Veins

Keywords

  • AAV vectors
  • Gene expression
  • Gene therapy
  • Hepatocytes
  • Second-strand DNA synthesis

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Self-complementary adeno-associated virus 2 (AAV)-T cell protein tyrosine phosphatase vectors as helper viruses to Improve transduction efficiency of conventional single-stranded AAV vectors in vitro and in vivo. / Zhong, Li; Chen, Linyuan; Li, Yanjun; Qing, Keyun; Weigel-Kelley, Kirsten A.; Chan, Rebecca; Yoder, Mervin; Srivastava, Arun.

In: Molecular Therapy, Vol. 10, No. 5, 11.2004, p. 950-957.

Research output: Contribution to journalArticle

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abstract = "Recombinant vectors based on adeno-associated virus type 2 (AAV) target the liver efficiently, but the transgene expression is limited to ∼5{\%} of hepatocytes. The lack of efficient transduction is due, in part, to the presence of a cellular protein, FKBP52, phosphorylated forms of which inhibit the viral second-strand DNA synthesis. We have documented that dephosphorylation of FKBP52 at tyrosine residues by the cellular T cell protein tyrosine phosphatase (TC-PTP) enhances AAV-mediated transduction in primary murine hematopoietic cells from TC-PTP-transgenic mice. We have also documented that AAV-mediated transduction is significantly enhanced in hepatocytes in TC-PTP-transgenic as well as in FKBP52-deficient mice because of efficient viral second-strand DNA synthesis. In this study, we evaluated whether co-infection of conventional single-stranded AAV vectors with self-complementary AAV-TC-PTP vectors leads to increased transduction efficiency of conventional AAV vectors in established human cell lines in vitro and in primary murine hepatocytes in vivo. We demonstrate here that scAAV-TC-PTP vectors serve as a helper virus in augmenting the transduction efficiency of conventional AAV vectors in vitro as well as in vivo which correlates directly with the extent of second-strand DNA synthesis of conventional single-stranded AAV vectors. Toxicological studies following tail-vein injections of scAAV-TC-PTP vectors in experimental mice show no evidence of any adverse effect in any of the organs in any of the mice for up to 13 weeks. Thus, this novel co-infection strategy should be useful in circumventing one of the major obstacles in the optimal use of recombinant AAV vectors in human gene therapy.",
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