SEM observations of intact and isolated proximal and collecting tubular cells from rat, rabbit and frog

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Abstract

The present study has examined both intact and isolated nephrons, particularly the proximal and collecting tubules. The isolated tubules were further treated by our HCI-collagenase digestion technique so that we would scan all surfaces of the tubular cells. Nephrons from rabbit, rat and frog were viewed. The cells of the proximal tubule from rat and rabbit were similar except for their basal surface. The early proximal tubule of the rat had numerous folds or plates that rested on the basement membrane while the rabbit possessed many basal villi. The proximal tubular cells of the frog were cuboidal in shape while the rat and rabbit had truncated cells. Changes in the cell surfaces features allowed us to characterize morphological segments. The collecting tubules were noted to have their characteristic two cell types, light and dark cells. The light cells were found to have little apical cell specialization but instead numerous basal infoldings. The dark cells possessed elaborate apical microplicae and few basal infoldings. The frog collecting tubule was lined by a principal cell and a flask cell.

Original languageEnglish (US)
Pages (from-to)312-323
Number of pages12
JournalBiomedical Research
Volume2
Issue numberSuppl.
StatePublished - Jan 1 1981

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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