Semaphorin-1a is required for Aedes aegypti embryonic nerve cord development

Morgan Haugen, Ellen Flannery, Michael Tomchaney, Akio Mori, Susanta K. Behura, David W. Severson, Molly Scheel

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Although mosquito genome projects have uncovered orthologues of many known developmental regulatory genes, extremely little is known about mosquito development. In this study, the role of semaphorin-1a (sema1a) was investigated during vector mosquito embryonic ventral nerve cord development. Expression of sema1a and the plexin A (plexA) receptor are detected in the embryonic ventral nerve cords of Aedes aegypti (dengue vector) and Anopheles gambiae (malaria vector), suggesting that Sema1a signaling may regulate mosquito nervous system development. Analysis of sema1a function was investigated through siRNA-mediated knockdown in A. aegypti embryos. Knockdown of sema1a during A. aegypti development results in a number of nerve cord phenotypes, including thinning, breakage, and occasional fusion of the longitudinal connectives, thin or absent commissures, and general distortion of the nerve cord. Although analysis of Drosophila melanogaster sema1a loss-of-function mutants uncovered many similar phenotypes, aspects of the longitudinal phenotypes differed between D. melanogaster and A. aegypti. The results of this investigation suggest that Sema1a is required for development of the insect ventral nerve cord, but that the developmental roles of this guidance molecule have diverged in dipteran insects.

Original languageEnglish
Article numbere21694
JournalPLoS One
Volume6
Issue number6
DOIs
StatePublished - 2011

Fingerprint

Semaphorins
Aedes
Aedes aegypti
ventral nerve cord
Culicidae
nerve tissue
Drosophila melanogaster
Phenotype
phenotype
Insects
Developmental Genes
Anopheles gambiae
insect development
Genes
neurodevelopment
dengue
Dengue
Regulator Genes
small interfering RNA
regulator genes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Haugen, M., Flannery, E., Tomchaney, M., Mori, A., Behura, S. K., Severson, D. W., & Scheel, M. (2011). Semaphorin-1a is required for Aedes aegypti embryonic nerve cord development. PLoS One, 6(6), [e21694]. https://doi.org/10.1371/journal.pone.0021694

Semaphorin-1a is required for Aedes aegypti embryonic nerve cord development. / Haugen, Morgan; Flannery, Ellen; Tomchaney, Michael; Mori, Akio; Behura, Susanta K.; Severson, David W.; Scheel, Molly.

In: PLoS One, Vol. 6, No. 6, e21694, 2011.

Research output: Contribution to journalArticle

Haugen, M, Flannery, E, Tomchaney, M, Mori, A, Behura, SK, Severson, DW & Scheel, M 2011, 'Semaphorin-1a is required for Aedes aegypti embryonic nerve cord development', PLoS One, vol. 6, no. 6, e21694. https://doi.org/10.1371/journal.pone.0021694
Haugen M, Flannery E, Tomchaney M, Mori A, Behura SK, Severson DW et al. Semaphorin-1a is required for Aedes aegypti embryonic nerve cord development. PLoS One. 2011;6(6). e21694. https://doi.org/10.1371/journal.pone.0021694
Haugen, Morgan ; Flannery, Ellen ; Tomchaney, Michael ; Mori, Akio ; Behura, Susanta K. ; Severson, David W. ; Scheel, Molly. / Semaphorin-1a is required for Aedes aegypti embryonic nerve cord development. In: PLoS One. 2011 ; Vol. 6, No. 6.
@article{140d00d3d0e24d3d9be710e1bf2871b9,
title = "Semaphorin-1a is required for Aedes aegypti embryonic nerve cord development",
abstract = "Although mosquito genome projects have uncovered orthologues of many known developmental regulatory genes, extremely little is known about mosquito development. In this study, the role of semaphorin-1a (sema1a) was investigated during vector mosquito embryonic ventral nerve cord development. Expression of sema1a and the plexin A (plexA) receptor are detected in the embryonic ventral nerve cords of Aedes aegypti (dengue vector) and Anopheles gambiae (malaria vector), suggesting that Sema1a signaling may regulate mosquito nervous system development. Analysis of sema1a function was investigated through siRNA-mediated knockdown in A. aegypti embryos. Knockdown of sema1a during A. aegypti development results in a number of nerve cord phenotypes, including thinning, breakage, and occasional fusion of the longitudinal connectives, thin or absent commissures, and general distortion of the nerve cord. Although analysis of Drosophila melanogaster sema1a loss-of-function mutants uncovered many similar phenotypes, aspects of the longitudinal phenotypes differed between D. melanogaster and A. aegypti. The results of this investigation suggest that Sema1a is required for development of the insect ventral nerve cord, but that the developmental roles of this guidance molecule have diverged in dipteran insects.",
author = "Morgan Haugen and Ellen Flannery and Michael Tomchaney and Akio Mori and Behura, {Susanta K.} and Severson, {David W.} and Molly Scheel",
year = "2011",
doi = "10.1371/journal.pone.0021694",
language = "English",
volume = "6",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "6",

}

TY - JOUR

T1 - Semaphorin-1a is required for Aedes aegypti embryonic nerve cord development

AU - Haugen, Morgan

AU - Flannery, Ellen

AU - Tomchaney, Michael

AU - Mori, Akio

AU - Behura, Susanta K.

AU - Severson, David W.

AU - Scheel, Molly

PY - 2011

Y1 - 2011

N2 - Although mosquito genome projects have uncovered orthologues of many known developmental regulatory genes, extremely little is known about mosquito development. In this study, the role of semaphorin-1a (sema1a) was investigated during vector mosquito embryonic ventral nerve cord development. Expression of sema1a and the plexin A (plexA) receptor are detected in the embryonic ventral nerve cords of Aedes aegypti (dengue vector) and Anopheles gambiae (malaria vector), suggesting that Sema1a signaling may regulate mosquito nervous system development. Analysis of sema1a function was investigated through siRNA-mediated knockdown in A. aegypti embryos. Knockdown of sema1a during A. aegypti development results in a number of nerve cord phenotypes, including thinning, breakage, and occasional fusion of the longitudinal connectives, thin or absent commissures, and general distortion of the nerve cord. Although analysis of Drosophila melanogaster sema1a loss-of-function mutants uncovered many similar phenotypes, aspects of the longitudinal phenotypes differed between D. melanogaster and A. aegypti. The results of this investigation suggest that Sema1a is required for development of the insect ventral nerve cord, but that the developmental roles of this guidance molecule have diverged in dipteran insects.

AB - Although mosquito genome projects have uncovered orthologues of many known developmental regulatory genes, extremely little is known about mosquito development. In this study, the role of semaphorin-1a (sema1a) was investigated during vector mosquito embryonic ventral nerve cord development. Expression of sema1a and the plexin A (plexA) receptor are detected in the embryonic ventral nerve cords of Aedes aegypti (dengue vector) and Anopheles gambiae (malaria vector), suggesting that Sema1a signaling may regulate mosquito nervous system development. Analysis of sema1a function was investigated through siRNA-mediated knockdown in A. aegypti embryos. Knockdown of sema1a during A. aegypti development results in a number of nerve cord phenotypes, including thinning, breakage, and occasional fusion of the longitudinal connectives, thin or absent commissures, and general distortion of the nerve cord. Although analysis of Drosophila melanogaster sema1a loss-of-function mutants uncovered many similar phenotypes, aspects of the longitudinal phenotypes differed between D. melanogaster and A. aegypti. The results of this investigation suggest that Sema1a is required for development of the insect ventral nerve cord, but that the developmental roles of this guidance molecule have diverged in dipteran insects.

UR - http://www.scopus.com/inward/record.url?scp=79959599801&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79959599801&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0021694

DO - 10.1371/journal.pone.0021694

M3 - Article

VL - 6

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 6

M1 - e21694

ER -