Sensitive assay for pimozide in human plasma using high-performance liquid chromatography with fluorescence detection: Application to pharmacokinetic studies

Thomas Kerbusch, Zeruesenay Desta, Nadia V. Soukhova, David Thacker, David A. Flockhart

Research output: Contribution to journalArticle

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Abstract

A method is described for the measurement of pimozide in human plasma using HPLC with fluorescence detection. The method is specific and sensitive in the range of concentrations seen in human plasma after conventional dosing (1-15 ng/ml) with a limit of quantification of 1 ng/ml. The calibration curves are linear for concentrations between 1 and 50 ng/ml. Within-day and inter-day coefficients of variation are less than 7.4% and 15.5%, respectively, at three concentrations of pimozide (2, 10 and 20 ng/ml). Intra-day and inter-day bias are less than 18.5% and 12.5%, respectively. A pharmacokinetic study conducted in a healthy volunteer administered 6 mg of pimozide orally demonstrates the utility of this method.

Original languageEnglish (US)
Pages (from-to)163-168
Number of pages6
JournalJournal of Chromatography B: Biomedical Applications
Volume694
Issue number1
DOIs
StatePublished - Jun 20 1997

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Keywords

  • Pimozide

ASJC Scopus subject areas

  • Chemistry(all)

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